The essentiality and involvement of Streptococcus intermedius histone-like DNA-binding protein in bacterial viability and normal growth

Molecular Microbiology
Dali LiuYoichiro Miyake

Abstract

Streptococcus intermedius histone-like DNA-binding protein (Si-HLP) is a homodimeric protein and, conserved with Escherichia coli HU, a well-documented nucleoid-associated protein (NAP). In E. coli, HU plays important roles as both structural and regulatory factors, but it is not essential for E. coli viability. Streptococcal HLP has been found to bind host cells and induce cytokine production, but its physiological role remains poorly defined. In the present study, using gene insertion knockout and tetracycline-regulated antisense RNA expression techniques, we determined whether Si-HLP is essential for bacterial viability and normal growth in S. intermedius. The Si-HLP-downregulated S. intermedius strain showed alterations in its morphology and surface properties. Downregulation of Si-HLP led to an expanded nucleoid to fill the intracellular space. Transcription levels of several genes, including virulence-associated factors, were found to be activated or repressed in the antisense Si-hlp RNA-expressing strain by real-time PCR and reverse-transcription PCR. Collectively, these data suggest that Si-HLP serves as an essential NAP governing the nucleoid architecture and controlling the gene transcription profile in S. intermedius.

References

Sep 1, 1990·Applied Microbiology and Biotechnology·M Geissendörfer, W Hillen
May 1, 1991·Journal of Bacteriology·A M DriP L Moreau
May 5, 1990·Journal of Molecular Biology·K KohnoF Imamoto
Aug 1, 1987·Applied and Environmental Microbiology·M C van LoosdrechtA J Zehnder
Jul 1, 1989·Journal of Bacteriology·O HuismanJ Rouvière-Yaniv
Jan 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·N E Dixon, A Kornberg
Apr 1, 1983·Infection and Immunity·G Westergren, J Olsson
Jan 1, 1982·Infection and Immunity·M W Stinson, E J Bergey
Feb 23, 1996·Journal of Molecular Biology·A BensaidJ Rouviere-Yaniv
Mar 1, 1997·Journal of Bacteriology·P Köhler, M A Marahiel
May 1, 2001·Biochemistry·K WojtuszewskiI Mukerji
May 22, 2002·Biochimica Et Biophysica Acta·Sabine Brantl
Oct 10, 2002·FEBS Letters·Remus Thei Dame, Nora Goosen
Oct 31, 2002·Research in Microbiology·Gianni ProssedaBianca Colonna
Apr 4, 2003·Current Opinion in Genetics & Development·Charles J Dorman, Padraig Deighan
May 16, 2003·The Journal of Biological Chemistry·Mitsumasa HashimotoYoke W Kow
Apr 23, 2004·Current Opinion in Structural Biology·Kerren K Swinger, Phoebe A Rice
Jul 28, 2004·Journal of Molecular Biology·Dror SagiJoel Stavans
Jan 8, 2005·Proceedings of the National Academy of Sciences of the United States of America·Galina PolekhinaMichael W Parker
May 24, 2005·Infection and Immunity·Bing Wang, Howard K Kuramitsu

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Citations

Feb 22, 2012·Journal of Bacteriology·Indranil Biswas, Saswat Sourav Mohapatra
May 8, 2015·Environmental Microbiology Reports·Mirian DomenechErnesto García
Jun 28, 2016·Extremophiles : Life Under Extreme Conditions·Anna C PapageorgiouConstantinos E Vorgias
Sep 30, 2017·FEMS Microbiology Letters·Anteneh Hailu AbebeItzhak Fishov
Dec 13, 2017·Frontiers in Molecular Biosciences·Jiahn-Haur LiaoShih-Hsiung Wu
Feb 10, 2010·Laboratory Investigation; a Journal of Technical Methods and Pathology·Ikuko HarutaJunji Yagi
Dec 17, 2014·Journal of Bacteriology·Morten KjosJan-Willem Veening
Feb 1, 2012·Prikladnaia biokhimiia i mikrobiologiia·A M AnuchinA S Kaprel'iants
Sep 22, 2019·International Journal of Molecular Sciences·Hiromichi YumotoYoichiro Miyake
Apr 7, 2016·Acta Crystallographica. Section F, Structural Biology Communications·Pierce O'NeilIndranil Biswas
Feb 14, 2015·Current Genetics·Ge Wang, Robert J Maier
Apr 18, 2018·Frontiers in Microbiology·María-José FerrándizAdela G de la Campa
Aug 28, 2021·International Journal of Molecular Sciences·Davide CampocciaCarla Renata Arciola

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