The Establishment and Characteristics of Rat Model of Atherosclerosis Induced by Hyperuricemia

Stem Cells International
Zhen LiuChanggui Li

Abstract

Epidemiological studies have identified hyperuricemia as an independent risk factor for cardiovascular disease. However, the mechanism whereby hyperuricemia causes atherosclerosis remains unclear. The objective of the study was to establish a new rat model of hyperuricemia-induced atherosclerosis. Wistar-Kyoto rats were randomly allocated to either a normal diet (ND), high-fat diet (HFD), or high-adenine diet (HAD), followed by sacrifice 4, 8, or 12 weeks later. Serum uric acid and lipid levels were analyzed, pathologic changes in the aorta were observed by hematoxylin and eosin staining, and mRNA expression was evaluated by quantitative real-time polymerase chain reaction. Serum uric acid and TC were significantly increased in the HAD group at 4 weeks compared with the ND group, but there was no significant difference in serum uric acid between the ND and HFD groups. Aorta calcification occurred earlier and was more severe in the HAD group, compared with the HFD group. Proliferating cell nuclear antigen, monocyte chemotactic factor-1, intercellular adhesion molecule-1, and vascular cell adhesion molecule-1 mRNA levels were increased in the HFD and HAD groups compared with the ND group. This new animal model will be a useful to...Continue Reading

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Citations

Apr 17, 2018·The Journal of Rheumatology·Iris J M DisveldMatthijs Janssen
Aug 13, 2020·International Journal of Experimental Pathology·Siân P CartlandMary M Kavurma
Aug 11, 2020·High Blood Pressure & Cardiovascular Prevention : the Official Journal of the Italian Society of Hypertension·Luiza Naujorks ReisCézane Priscila Reuter

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Methods Mentioned

BETA
PCR
reverse-transcription PCR

Software Mentioned

Pro Plus
Image
GraphPad Prism

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