The establishment of a chemically defined serum-free culture system for human dental pulp stem cells

Stem Cell Research & Therapy
Jingyi XiaoWeihua Guo

Abstract

The concept of establishing a dental stem cell (DSC) bank for oral and maxillofacial regeneration has become of great interest but it remains at a primitive stage. The routine application of serum-containing conditions for human DSC (hDSC) culture is in great controversy considering that the animal-originated serum can cause serious ethical concerns and lead to increasingly irrelevant variables, errors, and poor repeatability of experiment results. Thus, this study aimed to establish a safe, stable and efficient hDSC serum-free culturing system for future DSC bank usage. Dental pulp stem cells (DPSCs) from human permanent tooth pulp were isolated, expanded, passaged, and divided into two groups according to their culture conditions: group 1 was the serum-containing medium (SCM) group; and group 2 was the serum-free Essential 8 medium (E8) group. DPSCs were characterized first, followed by cell proliferation, pluripotency, and migration study in SCM and E8 medium. Human DPSCs (hDPSCs) in E8 medium demonstrated greater proliferation, pluripotency, migration ability and less apoptosis. hDPSCs could be successfully induced to the adipogenic, osteogenic, neurogenic, and chondrogenic lineages in E8 group. Real-time polymerase chain r...Continue Reading

References

Sep 1, 1991·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S HaradaE Ogata
Nov 1, 1996·Biotechnology Progress·D J MooneyB Rutherford
Jul 31, 2002·Journal of Dental Research·S GronthosS Shi
Apr 30, 2003·Proceedings of the National Academy of Sciences of the United States of America·Masako MiuraSongtao Shi
Feb 3, 2005·Transplantation Proceedings·R Y Calne
Sep 24, 2005·Journal of Cell Science·Ludovic VallierRoger A Pedersen
Oct 20, 2006·The New England Journal of Medicine·Dina Gould Halme, David A Kessler
Feb 9, 2007·Nature Biotechnology·Duncan E C BakerPeter W Andrews
Mar 3, 2007·Biochemical and Biophysical Research Communications·Tain-Hsiung ChenShih-Chieh Hung
Feb 26, 2008·Cell·George Q Daley, David T Scadden
Aug 18, 2009·Cell and Tissue Banking·Liesbeth TemmermanGuy A M De Pauw
Jul 16, 2010·Journal of Endodontics·Thais Miyuki HirataMarkus Haapasalo
Apr 9, 2011·Cell Stem Cell·Tasha KalistaChristopher Thomas Scott
Apr 12, 2011·Nature Methods·Guokai ChenJames A Thomson
Mar 15, 2012·Journal of Endodontics·Nikolay IshkitievMarkus Haapasalo
Apr 3, 2012·Journal of Cell Science·Maher AtariLuis Giner
Jul 4, 2012·Current Opinion in Genetics & Development·Tamer T Onder, George Q Daley
Sep 18, 2012·Archives of Oral Biology·Nobuyuki Kawashima
Dec 12, 2012·Cell Stem Cell·Naoki Oshimori, Elaine Fuchs
Jan 24, 2013·Expert Opinion on Biological Therapy·Sreedhar ThirumalaErik J Woods
Sep 4, 2013·Regenerative Medicine·Nikolay IshkitievToshio Imai
Sep 5, 2013·In Vitro Cellular & Developmental Biology. Animal·Xuan WangLiangjun Zhong
Jan 1, 2014·Frontiers in Physiology·Virginie BonnamainBrigitte Alliot-Licht
Apr 15, 2014·Cytotherapy·Shahla Hamza Al-SaqiCecilia Götherström
Jun 21, 2014·Archives of Oral Biology·Daniele LindemannLuciano Casagrande
Apr 11, 2015·Circulation Research·Vasileios Karantalis, Joshua M Hare
Apr 15, 2015·Stem Cells and Development·Petra HilkensIvo Lambrichts
Nov 8, 2016·Nature·Daniel P DeverMatthew H Porteus

❮ Previous
Next ❯

Citations

Jul 8, 2020·Stem Cells Translational Medicine·Oscar O Solis-CastroMarcelo N Rivolta
Dec 14, 2018·Stem Cell Research & Therapy·Xianrui YangDonghui Zhang
Sep 22, 2020·Nature Reviews. Materials·Elizabeth A Aisenbrey, William L Murphy
Sep 22, 2020·Dental Traumatology : Official Publication of International Association for Dental Traumatology·Mohammad AlansaryDawn Coates

❮ Previous
Next ❯

Methods Mentioned

BETA
FACS
flow cytometry
PCR

Software Mentioned

FlowJo

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.