PMID: 9554525Apr 29, 1998Paper

The establishment of a preoperative diagnosis of pancreatic carcinoma using cell specimens from pancreatic duct brushing with special attention to p53 mutations

Cancer
T IwaoG Kajiyama

Abstract

Previously, the authors reported that 82% of cases of pancreatic carcinoma were positive for p53 in cytologic specimens obtained by selective endoscopic pancreatic duct brushing (SEPB). However, there was an extreme discrepancy between the authors' data of p53 overexpression using cytologic specimens and other reports using surgically resected specimens. In this study, the authors demonstrate that p53 positive cells precisely reflect its gene mutations, and also establish systematic procedures for the preoperative diagnosis of patients with pancreatic carcinoma. The authors examined 44 cases of pancreatic carcinoma, 30 cases of chronic pancreatitis, and 9 cases of papillary adenoma. In all cases, pathologic diagnosis was made by surgery or autopsy. The conventional cytology and p53 immunocytology were performed simultaneously in the cell specimens obtained by SEPB. In the cases immunostained for p53, DNA was extracted selectively from p53 immunostained cells using a light microscope. p53 mutations in exons 5 to 8 were examined by direct sequencing. Forty of 44 pancreatic carcinomas (91%) were diagnosed correctly by the methods of conventional cytology associated with p53 immunocytology. p53 mutations were detected in 12 of 14 c...Continue Reading

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Citations

May 29, 2001·Journal of Clinical Pathology·C J StewartW R Murray
Feb 24, 2016·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Tatsuo HataMichiaki Unno
Jan 27, 2016·Langenbeck's Archives of Surgery·Rachel M GomesShailesh V Shrikhande
Dec 18, 2013·Seminars in Diagnostic Pathology·Claudio BellevicineGiancarlo Troncone
Jul 14, 2007·Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association·Georgios I PapachristouTodd H Baron
Aug 28, 2001·The Journal of Pathology·D CampaniG Bevilacqua
Nov 14, 2000·Diagnostic Cytopathology·C J Stewart, G M Burke
Aug 27, 2002·Advances in Anatomic Pathology·Andrew C HenkeMichael B Cohen
Apr 16, 2004·Pancreas·Norio SawabuYoshiharu Motoo
Jul 11, 2006·Drugs & Aging·Keiji HanadaYoshinori Kuroda
Jan 8, 2008·The American Journal of Gastroenterology·Sofia AsioliGianni Bussolati

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