PMID: 8601589Mar 1, 1996Paper

The estrogen-dependent c-JunER protein causes a reversible loss of mammary epithelial cell polarity involving a destabilization of adherens junctions

The Journal of Cell Biology
I FialkaH Beug

Abstract

Members of the epidermal growth factor (EGF) receptor family are known to be specifically involved in mammary carcinogenesis. As a nuclear target of activated receptors, we examined c-Jun in mammary epithelial cells. For this, we used a c-JunER fusion protein which was tightly controlled by estrogen. Activation of the JunER by hormone resulted in the transcriptional regulation of a variety of AP-1 target genes. Hormone-activated JunER induced the loss of epithelial polarity, a disruption of intercellular junctions and normal barrier function and the formation of irregular multilayers. These changes were completely reversible upon hormone withdrawal. Loss of epithelial polarity involved redistribution of both apical and basolateral proteins to the entire plasma membrane. The redistribution of E-cadherin and beta-catenin was accompanied by a destabilization of complexes formed between these two proteins, leading to an enrichment of beta-catenin in the detergent-soluble fraction. Uninduced cells were able to form three-dimensional tubular structures in collagen I gels which were disrupted upon JunER activation, leading to irregular cell aggregates. The JunER-induced disruption of tubular structures was dependent on active signalin...Continue Reading

References

Jan 1, 1992·Cold Spring Harbor Symposia on Quantitative Biology·K SimonsC Dotti
Jan 21, 1992·International Journal of Cancer. Journal International Du Cancer·D ReillyP A Andreasen
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·K RyderD Nathans
Jul 1, 1995·The Journal of Cell Biology·M S KinchK Burridge
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·S MunemitsuP Polakis
Feb 1, 1995·Current Opinion in Genetics & Development·P Polakis
Dec 1, 1994·The Journal of Cell Biology·J HülskenJ Behrens
May 1, 1994·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·W Birchmeier, C Birchmeier
Jan 1, 1993·Journal of Cell Science. Supplement·T P FlemingM Hay
Jan 1, 1993·Journal of Cell Science. Supplement·M CereijidoJ Valdés
Jan 1, 1993·Journal of Cell Science. Supplement·B M Gumbiner, P D McCrea
Jan 1, 1993·Journal of Cell Science. Supplement·M Edidin
Jan 1, 1993·Journal of Cell Science. Supplement·D F Woods, P J Bryant
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·R S ChaH Zarbl
Jun 2, 1994·The New England Journal of Medicine·E M Fish, B A Molitoris
Feb 1, 1994·Current Opinion in Genetics & Development·A Kazlauskas
Jan 1, 1993·Developmental Genetics·K H SchlingensiepenW Brysch
Dec 10, 1993·Science·B RubinfeldP Polakis
Oct 1, 1993·Endocrine Reviews·M Pfahl
Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·Z DongN H Colburn
Oct 1, 1993·The Journal of Cell Biology·M S BaldaJ M Anderson

❮ Previous
Next ❯

Citations

Aug 1, 1997·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·C Byrne
Apr 4, 1998·Electrophoresis·C PasqualiL A Huber
Dec 26, 2007·Pathology Oncology Research : POR·Seon Pil JinWoo Ho Kim
Apr 20, 2005·Journal of Mammary Gland Biology and Neoplasia·Kenna R Mills ShawJoan S Brugge
Jul 14, 1998·In Vitro Cellular & Developmental Biology. Animal·R MontesanoL Orci
Dec 27, 2006·Biochemical and Biophysical Research Communications·Roberto Montesano
Apr 21, 2004·Journal of Molecular Biology·N WickI Vietor
Sep 29, 2000·Biochemical Pharmacology·B BoyerN Edme
Dec 12, 2002·Steroids·Deborah A Lannigan
Sep 18, 1997·Journal of the American College of Surgeons·S E ReidE F Scanlon
Dec 21, 2006·Molecular Therapy : the Journal of the American Society of Gene Therapy·Saumya R ChaudhuriRichard L Hurwitz
Aug 10, 2012·Molecular Biology of the Cell·Maryline AllegraGeorge Mavrothalassitis
Feb 8, 2006·Genome Research·Stanislav VolikColin Collins
Apr 21, 2012·Breast Cancer Research : BCR·Rachel L DusekLaura D Attardi
Jan 11, 2014·PloS One·Taro IchimuraHideaki Fujita
Dec 10, 2013·European Journal of Cell Biology·Melanie KappelmannSilke Kuphal
May 28, 2005·Proceedings of the National Academy of Sciences of the United States of America·Caroline LassnigMathias Müller
Nov 14, 1997·Proceedings of the National Academy of Sciences of the United States of America·U KruseP K Vogt
Aug 2, 2014·Nature Communications·Mahdi MojallalLars Holmgren
Mar 22, 2000·The Journal of Cell Biology·S OliferenkoL A Huber
Sep 5, 2009·Current Opinion in Cell Biology·Michael E Feigin, Senthil K Muthuswamy
Feb 4, 2011·The Journal of Investigative Dermatology·Jane Y JinJennifer Y Zhang
Mar 28, 2009·Journal of Cellular Physiology·Jenifer R ProsperiKathleen H Goss

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.