The estrogen-regulated protein, TFF1, stimulates migration of human breast cancer cells

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Sara J PrestBruce R Westley

Abstract

The human trefoil protein TFF1 is a small cysteine-rich secreted protein that is frequently expressed in breast tumors under the control of estrogen. The function of TFF1 in breast cancer is unknown. To test the hypothesis that it promotes tumor dissemination, we produced recombinant TFF1 and assessed its ability to stimulate the movement of breast cancer cells by using in vitro wounding and migration assays. Recombinant TFF1 stimulated migration at concentrations of TFF1 found in culture medium. Migration of MCF-7 breast cancer cells, which secrete TFF1, was stimulated by lower concentrations of TFF1 than MDA MB231 cells that do not produce TFF1. Dimeric TFF1, linked by a disulfide bond, and monomeric TFF1 are produced by estrogen-responsive breast cancer cell lines. Recombinant TFF1 dimer was eightfold more potent than TFF1 monomer, implying that the interaction of TFF1 with its receptor is facilitated by dimerization. The majority of TFF1-stimulated migration resulted from chemotaxis, but dimeric TFF1 stimulated some chemokinesis. These results show that estrogens can stimulate the motility of breast cancer cells via the induction of TFF1 and suggest that one reason for the efficacy of hormonal therapies is their ability to ...Continue Reading

References

Dec 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·S SuemoriD K Podolsky
Oct 1, 1991·British Journal of Cancer·J A HenryB R Westley
Jul 1, 1989·Journal of Steroid Biochemistry·L R WisemanB R Westley
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M BerryP Chambon
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·G E Swaneck, J Fishman
Jan 1, 1988·Annals of the New York Academy of Sciences·L NegriP Melchiorri
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M C RioP Chambon
Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·F HauserW Hoffmann
Aug 1, 1993·The American Journal of Physiology·R Poulsom, N A Wright
Mar 28, 1997·Journal of Molecular Biology·V I PolshakovJ Feeney
Nov 26, 1997·The Journal of Pathology·F E May, B R Westley
Aug 26, 1998·The Journal of Pathology·T MarchbankR J Playford
Sep 18, 1998·Biochimica Et Biophysica Acta·S RibierasM C Rio
Apr 8, 1999·American Journal of Respiratory and Critical Care Medicine·A WiedeW Hoffmann
Jul 5, 2001·The American Journal of Pathology·M CrosierB R Westley

❮ Previous
Next ❯

Citations

Sep 12, 2012·Journal of Assisted Reproduction and Genetics·María-José EscribáMaría Sánchez-Serrano
Jan 11, 2011·Oncogene·M P WalkerG L Greene
Jul 17, 2004·American Journal of Respiratory Cell and Molecular Biology·Caroline E ChwieralskiWerner Hoffmann
May 21, 2014·International Journal of Medical Sciences·Milan MarkićevićDragica Nikolić-Vukosavljević
Feb 5, 2014·Clinical & Experimental Metastasis·Hassan Fazilaty, Parvin Mehdipour
Mar 10, 2010·Cellular and Molecular Life Sciences : CMLS·Alessandra ToscoLiberato Marzullo
Apr 6, 2012·Journal of Cancer Research and Clinical Oncology·Taweewun HunsawongPeti Thuwajit
Sep 3, 2010·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Lin Li, James R Davie
Jul 6, 2010·Biochemical and Biophysical Research Communications·Yong ZhangYun Zhang
Sep 9, 2010·Reproductive Biomedicine Online·Einat Shalom-PazSeang Lin Tan
Mar 9, 2010·Critical Reviews in Oncology/hematology·Raymond Manohar Anchan, Elizabeth Sarah Ginsburg
Jul 30, 2008·Experimental Cell Research·Akua K OduroFern E Murdoch
Jan 12, 2008·Advances in Enzyme Regulation·James R DavieXuemei Wang
Aug 13, 2015·Clinica Chimica Acta; International Journal of Clinical Chemistry·Ping XiaoRuirui Yang
Aug 2, 2007·Journal of Cellular Biochemistry·Shihua HeJames R Davie
Nov 16, 2004·Experimental Cell Research·Jian-Min SunJames R Davie
Jun 5, 2004·Peptides·Hannes KuttaFriedrich P Paulsen
Jun 5, 2004·Peptides·Shahin EmamiChristian Gespach
Jun 5, 2004·Peptides·Felicity E B MayBruce R Westley
Jul 25, 2006·Experimental Cell Research·Thorsten GorbaFrank Sieg
Apr 23, 2015·Endocrine-related Cancer·Felicity E B May, Bruce R Westley
Dec 10, 2013·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Miro KasumRebecca H Johnson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.