Abstract
Keratinocyte growth factor (KGF) regulates proliferation, differentiation, migration, and survival of different types of epithelial cells, including keratinocytes of the skin. To gain insight into the mechanisms underlying these multiple functions, we searched for KGF- regulated genes in keratinocytes. Using the differential display reverse transcriptase-PCR technology, we identified the gene encoding the estrogen-responsive B box protein (EBBP) which has as yet not been functionally characterized. The full-length murine and human EBBP cDNAs were cloned and fully sequenced. They were shown to encode 75-kDa proteins, which are mainly localized in the cytoplasm of keratinocytes in vitro and in vivo. In vivo, EBBP was found at high levels in the KGF- and epidermal growth factor-responsive basal keratinocytes of human skin, but the expression was down-regulated in the hyperthickened epithelium of skin wounds. Stable overexpression of EBBP in HaCaT keratinocytes did not affect the proliferation rate of the transfected cells, but enhanced the early differentiation process. These results suggest that the presence of EBBP in basal keratinocytes is important for the differentiation capacity of these cells, and that down-regulation of EB...Continue Reading
References
Feb 3, 1977·Nature·J G Rheinwald, H Green
Jan 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·T MikiS A Aaronson
Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·S WernerL T Williams
Aug 1, 1990·Journal of Cellular Physiology·C MarcheseS A Aaronson
May 11, 1991·Nucleic Acids Research·N C Andrews, D V Faller
Dec 1, 1990·The Journal of Cell Biology·E Fuchs
Mar 1, 1988·The Journal of Cell Biology·P BoukampN E Fusenig
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J S RubinS A Aaronson
Nov 1, 1995·The Journal of Experimental Medicine·C MarcheseS A Aaronson
May 1, 1995·Cell Biology International·J S RubinP W Finch
May 5, 1995·Cell·R A Weinberg
Nov 4, 1994·Science·S WernerL T Williams
Jul 1, 1993·The Journal of Investigative Dermatology·R TsuboiH Ogawa
May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·M MarikovskyM Klagsbrun
Mar 15, 1996·The Journal of Biological Chemistry·M D Hines, B L Allen-Hoffmann
Oct 4, 1996·The Journal of Biological Chemistry·S Frank, S Werner
Apr 4, 1997·Science·P Martin
Jun 12, 1997·Nature·D M HeeryM G Parker
Jan 14, 1998·Gene·M Madlener, S Werner
May 28, 1998·The Journal of Biological Chemistry·M IgarashiS A Aaronson
Jun 17, 1998·The EMBO Journal·N HuangR Losson
Oct 1, 1998·Cytokine & Growth Factor Reviews·S Werner
Nov 17, 1998·Molecular Endocrinology·H L LiuD A Zajchowski
Dec 29, 1998·Molecular Biology and Evolution·J HenryP Pontarotti
Jan 29, 1999·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·K L Borden
Dec 22, 1999·The Journal of Biological Chemistry·H Wakita, M Takigawa
May 2, 2001·The EMBO Journal·A ReymondA Ballabio
Jun 29, 2001·Differentiation; Research in Biological Diversity·M Torok, L D Etkin
Nov 1, 1990·The Journal of Investigative Dermatology·L E KingL B Nanney
Citations
Feb 14, 2013·Apoptosis : an International Journal on Programmed Cell Death·Patrick Y KimBelamy B Cheung
Aug 26, 2011·Nature Reviews. Immunology·Caroline JefferiesRowan Higgs
Aug 24, 2010·Oncogene·G M MarshallB B Cheung
Apr 1, 2006·Cell Death and Differentiation·C MundingH-D Beer
Jul 25, 2006·Oncogene·S BraunS Werner
Mar 5, 2010·Molecular Medicine·Daniela SpanoAchille Iolascon
Feb 24, 2016·Cancer Letters·Patrick Y KimBelamy B Cheung
May 1, 2007·Toxicology and Applied Pharmacology·Scott W BurchielTudor I Oprea
Oct 25, 2013·Journal of Cellular and Molecular Medicine·Paul W FinchCatherine L Farrell
Oct 20, 2011·The Journal of Pathology·Belamy B CheungGlenn M Marshall
May 11, 2011·The Journal of Pathology·Adam GiangrecoSam M Janes
Feb 21, 2013·Cell Cycle·Jessica L BellBelamy B Cheung
Jun 30, 2007·Current Biology : CB·Laurence FeldmeyerHans-Dietmar Beer
Sep 7, 2014·Cytokine & Growth Factor Reviews·Siobhán Smith, Caroline Jefferies
Nov 7, 2016·Fish & Shellfish Immunology·Yepin YuQiwei Qin
Jul 14, 2005·Maturitas·Antovska Vesna, Basheska Neli
Jan 4, 2018·Nutrients·Pei-Hui LinJianjie Ma
Oct 21, 2005·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Germana Meroni, Graciana Diez-Roux
Apr 26, 2006·The Journal of Biological Chemistry·Belamy B CheungGlenn M Marshall
Oct 22, 2014·Oncotarget·Selina K SuttonGlenn M Marshall
Feb 8, 2013·Experimental Dermatology·Sarah LeightonScott N Byrne
Jun 20, 2021·Cell Metabolism·Lin WangKe-Feng Dou
Aug 27, 2021·Cell and Tissue Research·Qianwei Cui, Li Yan
Feb 3, 2011·Archives of Biochemistry and Biophysics·Claudia SkazikJens M Baron