PMID: 8460141Mar 15, 1993Paper

The eukaryotic host factor that activates exoenzyme S of Pseudomonas aeruginosa is a member of the 14-3-3 protein family

Proceedings of the National Academy of Sciences of the United States of America
H FuR J Collier

Abstract

Exoenzyme S (ExoS), which has been implicated as a virulence factor of Pseudomonas aeruginosa, catalyzes transfer of the ADP-ribose moiety of NAD+ to many eukaryotic cellular proteins. Its preferred substrates include Ras and several other 21- to 25-kDa GTP-binding proteins. ExoS absolutely requires a ubiquitous eukaryotic protein factor, termed FAS (factor activating ExoS), for enzymatic activity. Here we describe the cloning and expression of a gene encoding FAS from a bovine brain cDNA library and demonstrate that purified recombinant FAS produced in Escherichia coli activates ExoS in a defined cell-free system. The deduced amino acid sequence of FAS shows that the protein (245 residues, calculated molecular mass 27,743 Da) belongs to a highly conserved, widely distributed eukaryotic protein family, collectively designated as 14-3-3 proteins. Various functions have been reported for members of the 14-3-3 family, including phospholipase A2 activity and regulation of tyrosine hydroxylase, tryptophan hydroxylase, and, possibly, protein kinase C activities. Identification of FAS as a 14-3-3 protein establishes an additional function for this family of proteins--the activation of an exogenous ADP-ribosyltransferase. Elucidation o...Continue Reading

References

Jul 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·B H IglewskiE S Maxwell
Sep 1, 1992·The Plant Journal : for Cell and Molecular Biology·J BrandtD B Collinge
May 15, 1992·Biochemical and Biophysical Research Communications·G J MartensE H Danen
Dec 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·G LuR J Ferl
Mar 26, 1991·Biochimica Et Biophysica Acta·P J Nielsen
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·T StearnsR A Kahn
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·T IchimuraY Takahashi
Apr 1, 1985·Canadian Journal of Microbiology·T I Nicas, B H Iglewski
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R H AebersoldS B Kent
May 5, 1986·Journal of Molecular Biology·F W Studier, B A Moffatt
Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies
May 1, 1982·Journal of Neurochemistry·P F BostonR J Thompson
Oct 1, 1993·Journal of Anatomy·R D BurgoyneT R Cheek

❮ Previous
Next ❯

Citations

Oct 1, 1996·Journal of Molecular Evolution·W Wang, D C Shakes
Mar 1, 1994·Molecular and Biochemical Parasitology·P S ShenJ Samuelson
Jul 21, 1994·Biochimica Et Biophysica Acta·Y PatelA Aitken
Jan 15, 1996·FEMS Microbiology Letters·J Goranson, D W Frank
Mar 17, 2004·Biochemical and Biophysical Research Communications·Miki TamuraTeiji Sawa
Jun 19, 2001·Biochimica Et Biophysica Acta·H YangH Fu
Jun 19, 2002·Trends in Biochemical Sciences·Richard A KahnCraig R Roy
Aug 15, 2009·Nature Reviews. Microbiology·Alan R Hauser
Aug 18, 2004·Nature Structural & Molecular Biology·Jianjun SunJoseph T Barbieri
Oct 18, 2002·European Journal of Biochemistry·Maria L HenrikssonBengt Hallberg
Sep 13, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jing ZhaoHaian Fu
Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·R G Martin, J L Rosner
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·D S ConklinD Beach
Oct 24, 1995·Proceedings of the National Academy of Sciences of the United States of America·N Bonnefoy-BérardA Altman
Dec 5, 1995·Proceedings of the National Academy of Sciences of the United States of America·D GelperinS Lemmon
Mar 28, 2009·Critical Care Medicine·Jeffrey L VeesenmeyerJordi Rello
Mar 9, 1994·Annals of the New York Academy of Sciences·R D BurgoyneD Roth
Sep 27, 2002·Applied and Environmental Microbiology·Jörg SchirmerKlaus Aktories
Sep 13, 2006·Infection and Immunity·Jinghua JiaShouguang Jin
Aug 14, 2001·Infection and Immunity·A M BarbieriM Vidal
Aug 23, 2005·Infection and Immunity·Claudia L RochaJoan C Olson
Jan 26, 2010·Journal of Bacteriology·Katherine M SchmalzerDara W Frank
Jun 22, 2001·Journal of Bacteriology·V Finck-Barbançon, D W Frank
Jun 1, 1996·Annual Review of Plant Physiology and Plant Molecular Biology·Robert J. Ferl
Sep 13, 2008·Annual Review of Microbiology·Qing Deng, Joseph T Barbieri
Jun 3, 2000·Annual Review of Pharmacology and Toxicology·H FuS C Masters
Nov 15, 2008·Biological Chemistry·Corinna OttmannChristian Ottmann
Dec 17, 2008·Future Microbiology·Sigrid D AuweterB Brett Finlay
Feb 21, 2014·International Journal of Molecular Sciences·Guodong HuJihua Wang
Jan 7, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Mei LiuJian-Ying Zhang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.