The eukaryotic tRNA-guanine transglycosylase enzyme inserts queuine into tRNA via a sequential bi-bi mechanism

Chemical Communications : Chem Comm
Mashael A AlqasemVincent P Kelly

Abstract

Eukaryotic tRNA-guanine transglycosylase (TGT) - an enzyme recently recognised to be of potential therapeutic importance - catalyses base-exchange of guanine for queuine at the wobble position of tRNAs associated with 4 amino acids via a distinct mechanism to that reported for its eubacterial homologue. The presence of queuine is unequivocally required as a trigger for reaction between the enzyme and tRNA and exhibits cooperativity not seen using guanine as a substrate.

References

Jul 7, 1992·Biochimica Et Biophysica Acta·U GündüzJ R Katze
Jan 1, 1983·Progress in Nucleic Acid Research and Molecular Biology·S Nishimura
Jan 13, 1997·Biochemical and Biophysical Research Communications·T Marks, W R Farkas
Apr 17, 2003·Bioorganic Chemistry·Dirk Iwata-Reuyl
Jul 25, 2003·Bioorganic Chemistry·DeeAnne M Goodenough-Lashua, George A Garcia
Nov 23, 2006·RNA Biology·Chandramani PathakManjula Vinayak
May 15, 2007·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Harvey WongSanjeev Kumar
May 6, 2009·The Journal of Biological Chemistry·Coilin BolandVincent P Kelly
Apr 14, 2011·The Journal of Biological Chemistry·Tatsiana RakovichVincent P Kelly
Aug 22, 2012·Annual Review of Genetics·Basma El YacoubiValérie de Crécy-Lagard
Mar 4, 2014·Trends in Molecular Medicine·Adrian Gabriel TorresLluis Ribas de Pouplana
Jun 10, 2014·ACS Chemical Biology·Rémi ZallotValérie de Crécy-Lagard
Apr 18, 2015·Nutrients·Claire FergusVincent P Kelly
May 26, 2017·Frontiers in Molecular Neuroscience·Andrea BednářováNatraj Krishnan
Aug 11, 2018·The EMBO Journal·Francesca TuortoFrank Lyko
Feb 5, 2019·Nucleic Acids Research·Martin MüllerAnn E Ehrenhofer-Murray

❮ Previous
Next ❯

Citations

Jul 10, 2021·RNA Biology·Katharina SieversRalf Ficner

❮ Previous
Next ❯

Methods Mentioned

BETA
equilibrium dialysis

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.