The Eurocine L3 adjuvants with subunit influenza antigens induce protective immunity in mice after intranasal vaccination

Vaccine
Pernilla PeterssonAke Lundkvist

Abstract

The immunogenicity and protective efficacy in mice of intranasally (i.n.) administrated influenza subunit antigens together with lipid-based adjuvants (Eurocine) were compared to those of subcutaneous (s.c.) immunisation. Influenza hemagglutination inhibition (HAI) and ELISA IgG titers were similar in the group's vaccinated s.c. and after i.n. vaccination with adjuvants. The virus-specific IgA levels in serum were higher after vaccination i.n. with adjuvant than after s.c immunisation. Virus-specific IgA was measurable in nasal washings only after i.n vaccinations, with and without adjuvants. Thus, i.n. vaccination with the endogenous non-toxic, lipid adjuvants induced equal or stronger antibody responses as compared to s.c. immunisation with the same antigen. We further analysed the protective efficacy against virus challenge in a mouse model. A subunit antigen preparation of the A/New/Caledonia/20/99 strain was used for vaccination of NMRI mice with different combinations of adjuvants. The mice were challenged i.n. with 6.5 tissue culture infectious doses(50) of homologous virus and sacrificed 3 days later. Since the virus is not lethal in mice, the protective efficacy was measured by quantitative, real-time PCR on pulmonary ...Continue Reading

References

Apr 11, 2003·Vaccine·George Kemble, Harry Greenberg
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Jan 24, 2007·Vaccine·Marie Paule KienyKeiji Fukuda
Oct 21, 2009·Vaccine·Guus F RimmelzwaanAlbert D M E Osterhaus

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Citations

Nov 29, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·J F S MannP F McKay
Jul 5, 2016·Scientific Reports·Masayuki HayashiKen J Ishii
Mar 31, 2012·Clinical and Vaccine Immunology : CVI·Luigi BuonaguroFranco M Buonaguro
Jul 12, 2013·ACS Chemical Biology·Francesco Berti, Roberto Adamo

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