The evaluation and implementation of Direct Analysis in Real Time quadrupole time-of-flight tandem mass spectrometry for characterization and quantification of geniposide in Re Du Ning Injections

Rapid Communications in Mass Spectrometry : RCM
Yan-Jing LiXue Wang

Abstract

The Direct Analysis in Real Time (DART) ionization source coupled with a quadrupole time-of-flight tandem mass spectrometry (Q-TOF MS/MS) system has the capability to desorb analytes directly from samples from complex Chinese herbal preparations without sample cleanup or chromatographic separation. In this work, a method based on DART/Q-TOF MS/MS has been developed for rapid determination of geniposide present in 'Re Du Ning Injections', a Chinese herbal preparation. The method has been evaluated for both qualitative and quantitative analysis of geniposide in Re Du Ning Injections. Variables including polarity for ion detection, DART gas heater temperature, matrix effect and sample presentation speed were investigated. The quantitative method was validated with respect to linearity, sensitivity, repeatability, precision and accuracy by using both internal and external standards. A comparison of the results obtained using the DART-based method was made with those obtained using a conventional High-Performance Liquid Chromatography/Diode-Array Detector (HPLC/DAD) by analyzing geniposide in four batches of Re Du Ning Injections. The DART/Q-TOF MS/MS-based method provides a rapid, efficient and powerful method to analyze compounds ...Continue Reading

References

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Feb 4, 2010·Analytical and Bioanalytical Chemistry·Manuela HaunschmidtRobert Hertsens
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Citations

Aug 6, 2013·Analytical and Bioanalytical Chemistry·Sonja KriegerOliver J Schmitz
Jan 11, 2013·Chemical Reviews·María Eugenia MongeFacundo M Fernández
Dec 19, 2013·Journal of the American Society for Mass Spectrometry·Lu WangHaibin Qu
Jul 30, 2014·Analytical and Bioanalytical Chemistry·Anastasia AlbertCarsten Engelhard
Nov 5, 2014·BMC Complementary and Alternative Medicine·Haixing YangYonghua Wang
Mar 10, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Brajesh KumarKleber Andrade
Sep 10, 2015·Rapid Communications in Mass Spectrometry : RCM·Matthew CurtisFacundo M Fernández
May 2, 2015·Journal of the American Society for Mass Spectrometry·Liya ZhangYiyang Dong
Jan 1, 2021·BioMed Research International·Fuda XieJiangyong Gu

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