The evaluation of an identification algorithm for Mycobacterium species using the 16S rRNA coding gene and rpoB

International Journal of Mycobacteriology
Yuko Kazumi, Satoshi Mitarai

Abstract

Conventional biochemical tests are the standard for the identification of Mycobacterium species, but molecular identifications are becoming more prevalent. The rpoB gene encodes the β-subunit of RNA polymerase and is utilized for the identification of Mycobacterium species. In the present study, a stepwise Mycobacterium species identification algorithm using the 16S rRNA encoding gene and rpoB analysis was evaluated for its effectiveness. A total of 172 clinical Mycobacterium isolates were tested, and concordant results were obtained with 108 strains by using the conventional method and molecular methods (AccuProbe or DDH method). In these 108 strains, 4 strains were identified by 16S rRNA gene analysis, but rpoB indicated no identical Mycobacterium species with more than 99% similarity. The remaining 64 strains were not identified by conventional method and commercial kits. Forty-two showed concordant results with 16S rRNA and rpoB analysis, and 13 strains were identified by 16S rRNA gene analysis although rpoB indicated no identical Mycobacterium species. On the other hand, 4 strains included 2 strains of Gordona and 2 strains of M. celatum type II which were identified by rpoB but not by 16S rRNA gene analysis. Finally, 5 st...Continue Reading

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Citations

Nov 21, 2008·Journal of Clinical Microbiology·Stephen J McBrideDavid J Holland
Jul 17, 2015·BioMed Research International·Ali Akbar VelayatiMehdi Mirsaeidi
Jun 1, 2014·International Journal of Mycobacteriology·Ines JoaoLuisa Jordao
Nov 6, 2013·The American Journal of Tropical Medicine and Hygiene·Ali Akbar VelayatiSven Hoffner
Apr 21, 2018·Genome Announcements·A BouamM Drancourt
Sep 26, 2017·Internal Medicine·Hiroyuki FujikuraKeiichi Mikasa

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