The evaluation of lyophilized polymer matrices for administering recombinant human bone morphogenetic protein-2

Pharmaceutical Development and Technology
S S DuggiralaP P DeLuca

Abstract

Novel unitary devices, prepared by lyophilization of viscous solutions of sodium carboxymethylcellulose (CMC) and methylcellulose (MC), were evaluated as sustained-release delivery systems for recombinant human bone morphogenetic protein-2 (rhBMP-2). In vitro characterization of the unitary devices, which contained rhBMP-2-loaded poly (d,l lactide-co-glycolide) (PLGA) bioerodible particles (BEPs), was conducted over a 2-month period. Determinations included buffer uptake, mass and molecular weight loss and rhBMP-2 release from the unitary devices. CMC devices imbibed approximately 16 times their weight of buffer, while with MC, equilibrium uptake was approximately 6 times the dry weight of the devices. Overall mass loss percentages were approximately 55 and 35%, respectively, for CMC and MC devices. rhBMP-2 release from the devices was essentially a triphasic process: an initial phase during which "free" protein (rhBMP-2 present on the surface and within the pores of the PLGA BEPs) was released, a lag period during which no release was discerned, and then release of "bound" rhBMP-2 (protein adsorbed to the BEPs). The release of bound protein correlated with the mass loss of the polymer which began after 3 weeks. Release from th...Continue Reading

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Citations

Jan 20, 2004·AAPS PharmSciTech·J A SchrierP P DeLuca
Dec 1, 1999·Pharmaceutical Development and Technology·J A Schrier, P P DeLuca
Jan 7, 1999·Drug Development and Industrial Pharmacy·R JainC T Rhodes
Jul 29, 2010·Journal of Biomedical Materials Research. Part a·Hermann AgisReinhard Gruber
Apr 11, 2012·Acta Biomaterialia·Noel DavisonFlorence Barrere-de Groot
Jun 26, 1998·Journal of Biomedical Materials Research·D A PuleoP P DeLuca
Sep 29, 2018·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Ayumi SakamotoMuneki Honnami
Jul 22, 2005·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Stephen D CookLaura P Patron
Apr 20, 2007·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Mark A ReynoldsMichael D Rohrer
May 22, 2019·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Melanie CoathupGordon Blunn
Feb 23, 1999·Pharmaceutical Development and Technology·W P YeF S Du

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