The evaluation of the transport medium for extracted premolars prior to cryopreservation: an in vitro study

Cell and Tissue Banking
Noëmi M C De RooGuy A M De Pauw

Abstract

Autotransplantation is a versatile technique for the replacement of a missing tooth and cryopreservation can expand its scope. The aim of this in vitro study is to compare the antimicrobial effect of different transport protocols on procured teeth prior to cryopreservation. Streptococcus oralis biofilms were grown on ten sterile premolars, incubated for 48 h and subjected to the following transport procedures: an untreated (contaminated) control group, a group rinsed with phosphate buffered saline (PBS), a group transported in PBS, a group transported in Dulbecco's Modified Eagle's medium (DMEM) supplemented with fetal calf serum (FCS), and a group transported in DMEM supplemented with FCS and antibiotics (AB). The effect of cryopreservation as such, as well as the combination with a transport medium (DMEM + FCS + AB) on the contamination was also tested. The surviving bacteria were harvested, and determined by plate counting. There was no significant reduction in contamination after rinsing the tooth, after transport in PBS or after transport in DMEM with FCS. Significant reductions were observed for transport in DMEM with AB when compared to the control group (p = 0.003). Cryopreservation as such reduced the biofilm significa...Continue Reading

References

Apr 1, 1982·International Journal of Oral Surgery·M B Comfort
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May 24, 2001·Journal of Bacteriology·B J PasterF E Dewhirst
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May 9, 2006·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·Liesbeth TemmermanLuc R Dermaut
Aug 26, 2006·Periodontology 2000·Bruce J PasterFloyd E Dewhirst
Dec 27, 2007·The Canadian Journal of Infectious Diseases = Journal Canadien Des Maladies Infectieuses·Mahmoud Rouabhia

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BETA
FCS

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GPower
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