The evolutionarily conserved family of cyanovirin-N homologs: structures and carbohydrate specificity

Structure
Leonardus M I KoharudinAngela M Gronenborn

Abstract

Solution structures for three members of the recently discovered cyanovirin-N (CV-N) homolog family of lectins have been determined. Cyanovirin-N homologs (CVNHs) from Tuber borchii, Ceratopteris richardii, and Neurospora crassa, representing each of the three phylogenetic groups, were selected. All proteins exhibit the same fold, and the overall structures resemble that of the founding member of the family, CV-N, albeit with noteworthy differences in loop conformation and detailed local structure. Since no data are available regarding the proteins' function or their natural ligands, extensive carbohydrate-binding studies were conducted. We delineated ligand-binding sites on all three proteins by nuclear magnetic resonance and identified which sugars interact by array screening. The number and location of binding sites vary for the three proteins, and different ligand specificities exist. Potential physiological roles for two family members, TbCVNH and NcCVNH, were probed in nutrition deprivation experiments that suggest a possible involvement of these proteins in lifestyle-related responses.

References

Nov 1, 1995·Journal of Biomolecular NMR·F DelaglioA Bax
Dec 1, 1996·Journal of Biomolecular NMR·R A LaskowskiJ M Thornton
Feb 21, 1998·Trends in Biotechnology·G M Clore, A M Gronenborn
Jul 17, 1998·Nature Structural Biology·C A BewleyA M Gronenborn
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
Mar 28, 2002·Journal of the American Chemical Society·Brendan S KelleyCarole A Bewley
Jul 12, 2002·The Journal of Biological Chemistry·Istvan BotosAlexander Wlodawer
Jan 31, 2003·Proteins·Jens P LingeMichael Nilges
Apr 30, 2003·Antiviral Research·Laura G BarrientosMichael R Boyd
Jul 25, 2003·Antimicrobial Agents and Chemotherapy·Barry R O'KeefeMichael R Boyd
Mar 6, 2004·AIDS Research and Human Retroviruses·Che-Chung TsaiMichael R Boyd
Aug 26, 2004·Journal of Molecular Biology·Naomi Siew, Daniel Fischer
Oct 19, 2004·Annual Review of Microbiology·Patricia L Lakin-Thomas, Stuart Brody
Jan 11, 2005·Mini Reviews in Medicinal Chemistry·Laura G Barrientos, Angela M Gronenborn
May 16, 2006·Fungal Genetics and Biology : FG & B·Barbara MontaniniSimone Ottonello
Jun 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·Hildur V ColotJay C Dunlap
Apr 14, 2007·BMC Bioinformatics·Barbara LazzariAngelo Viotti
Sep 1, 1994·Journal of Biomolecular NMR·B A Johnson, R A Blevins

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Citations

Apr 8, 2011·Annual Review of Biochemistry·Cory D Rillahan, James C Paulson
May 29, 2009·Nucleic Acids Research·Smriti ShridharGitanjali Yadav
Oct 22, 2010·The Journal of Biological Chemistry·Leonardus M I KoharudinAngela M Gronenborn
Jan 21, 2010·Yi chuan = Hereditas·Xiao-Qiong QiTing Wang
Jan 12, 2011·Virologica Sinica·Hong YuWen-qing Zhang
Apr 10, 2015·International Journal of Molecular Sciences·Mohamed Ali Abol HassanJoe Tiralongo
Nov 2, 2015·Applied Microbiology and Biotechnology·Jerica SabotičMarkus Künzler
Mar 21, 2009·Trends in Microbiology·Ronnie de Jonge, Bart P H J Thomma
Mar 3, 2011·Proteins·Elena MateiAngela M Gronenborn
Oct 2, 2012·Biopolymers·Leonardus M I Koharudin, Angela M Gronenborn
Jul 30, 2009·Proteins·Leonardus M I KoharudinAngela M Gronenborn
Jan 24, 2009·Current Opinion in Structural Biology·Angela M Gronenborn
Mar 13, 2014·Biophysical Journal·Ashini BoliaGiovanna Ghirlanda
Oct 13, 2012·Journal of the American Chemical Society·Yukiji K Fujimoto, David F Green
Apr 25, 2017·International Journal of Biological Macromolecules·Ram Sarup SinghJohn F Kennedy
Jul 13, 2016·The Journal of Biological Chemistry·Elena MateiAngela M Gronenborn
Jan 13, 2019·International Journal of Molecular Sciences·Annick BarrePierre Rougé
Oct 18, 2020·Proteins·Andrei Santos SiqueiraEvonnildo Costa Gonçalves

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