The evolutionarily conserved HtrA is associated with stress tolerance and protein homeostasis in the halotolerant cyanobacterium Halothece sp. PCC7418

Extremophiles : Life Under Extreme Conditions
Tanutcha PatipongRungaroon Waditee-Sirisattha

Abstract

The HtrA protein family represents an important class of serine proteases that are widely distributed across taxa. These evolutionarily conserved proteins are crucial for survival and function as monitors of protein synthesis during various stresses. Here, we performed gene expression analysis of the entire set of putative serine protease genes in Halothece sp. PCC7418 under salt stress conditions. The gene-encoding HtrA2 (H3553) was highly upregulated. This gene was cloned and functionally characterized, and its sub-cellular localization was determined. The recombinant H3553 protein (rH3553) displayed a pH optimum of 8.0, remained stable at 45 °C, and its proteolytic activity was not affected by salts. H3553 completely degraded the unfolded model protein, β-casein. In contrast, the folded model substrates (lysozyme or BSA) were not degraded by rH3553. Denaturation of BSA at a high temperature significantly increased its degradation by rH3553. H3553 was detected in the soluble protein fraction as well as the plasma membrane and thylakoid membrane fractions. Interestingly, the majority of H3553 was present in the plasma membrane under salt and heat stress conditions. Thus, H3553 resides in multiple sub-cellular locations and its...Continue Reading

References

Oct 12, 1999·Molecular & General Genetics : MGG·J Skórko-GlonekB Lipinska
Jan 10, 2002·Biochemical and Biophysical Research Communications·Yu KanesakiNorio Murata
Mar 3, 2004·Molecular & Cellular Proteomics : MCP·Fang HuangBirgitta Norling
Jul 29, 2005·Photosynthesis Research·Tove JansénPirkko Mäenpää
Oct 7, 2006·Biochimica Et Biophysica Acta·Valentina CecariniJeffrey N Keller
Nov 17, 2007·Genes & Development·Noboru Mizushima
Feb 9, 2008·Cellular and Molecular Life Sciences : CMLS·M J Page, E Di Cera
Aug 14, 2008·Proceedings of the National Academy of Sciences of the United States of America·Jiansen JiangSen-Fang Sui
Oct 7, 2008·FEMS Microbiology Reviews·Amel LatifiCheng-Cai Zhang
Apr 21, 2009·Molecular Biology and Evolution·Morgan N PriceAdam P Arkin
Aug 22, 2009·Research in Microbiology·Michael MeltzerMichael Ehrmann
Aug 25, 2009·Applied and Environmental Microbiology·Fuminori FukayaTeruhiro Takabe
Oct 3, 2012·PloS One·Irma N RobertsChristiane Funk
Jan 19, 2013·Molecular Biology and Evolution·Kazutaka Katoh, Daron M Standley
Oct 24, 2013·Archaea : an International Microbiological Journal·Christopher J ReedCaryn Evilia
Feb 24, 2015·Journal of Molecular Biology·Jan-Ulrik DahlUrsula Jakob
May 20, 2015·Computational and Structural Biotechnology Journal·Nivedita Rai, Amutha Ramaswamy
Jun 13, 2015·Current Opinion in Microbiology·Shiladitya DasSarma, Priya DasSarma
Feb 28, 2016·Molecular Biology and Evolution·Jaime Huerta-CepasPeer Bork
Jun 3, 2016·Frontiers in Plant Science·Otilia CheregiChristiane Funk
Dec 16, 2016·Microbiology and Molecular Biology Reviews : MMBR·Samuel H Becker, K Heran Darwin
May 2, 2017·Annual Review of Biochemistry·Ivan Dikic

❮ Previous
Next ❯

Citations

Aug 5, 2021·Plant, Cell & Environment·Chananwat KortheerakulRungaroon Waditee-Sirisattha

❮ Previous
Next ❯

Datasets Mentioned

BETA
PCC7942

Methods Mentioned

BETA
reverse transcription-PCR
PCR
gel filtration
protease assay
salt stress
electrophoresis

Software Mentioned

PerlPrimer
GraphPad Prism7
ETE3
Image [UNK]
SignalP
FastTree
MAFFT
Compute pI / Mw

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.