Chemogenetic activation of astrocytes in the hippocampus and cortex changes the transcriptome of microglia and other cell types.

BioRxiv : the Preprint Server for Biology
S. PhiltjensJungsu Kim


Astrocytes are the most common glial cell type in the brain, yet, it is still not clear how their activation affects the transcriptome of other brain cells such as microglia and neurons. Engineered G protein-coupled receptors called Designer Receptors Exclusively Activated by Designer Drugs (DREADDS) make it possible to selectively activate specific cell types, such as neurons and astrocytes. By combining the selective activation of astrocytes with single cell RNA sequencing, we were able to study transcriptional changes that occur in response to the activation of astrocytes at the single cell level. Interestingly, our data shows that long-term activation of astrocytes in healthy mice results in dramatic alteration in the transcriptome of astrocytes and microglia. Genes that were differentially expressed in these Gq-DREADD-activated astrocytes were involved in neurogenesis and low density lipoprotein particle biology, while those in the microglia were involved in the response to lipoproteins, and the migration and chemotaxis of immune cells. Furthermore, network analysis showed that Gq-DREADD-mediated activation in astrocytes resulted in an upregulation of genes involved in the G protein-coupled receptor signaling pathway and c...Continue Reading

Related Concepts

Caenorhabditis elegans
Muscle Cells
Muscle Innervation, Function
Transcription, Genetic
Myocytes, Smooth Muscle
Set of Muscles
Contraction (Finding)

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.

Related Papers

Current Biology : CB
Florian Raible, Kristin Tessmar-Raible
Tidsskrift for den Norske lægeforening : tidsskrift for praktisk medicin, ny række
© 2020 Meta ULC. All rights reserved