The evolving interface between synthetic biology and functional metagenomics.

Nature Chemical Biology
Eric van der HelmMorten O A Sommer

Abstract

Nature is a diverse and rich source of bioactive pathways or novel building blocks for synthetic biology. In this Perspective, we describe the emerging research field in which metagenomes are functionally interrogated using synthetic biology. This approach substantially expands the set of identified biological activities and building blocks. In reviewing this field, we find that its potential for new biological discovery is dramatically increasing. Functional metagenomic mining using genetic circuits has led to the discovery of novel bioactivity such as amidases, NF-κB modulators, naphthalene degrading enzymes, cellulases, lipases and transporters. Using these genetic circuits as a template, improvements are made by designing biosensors, such as in vitro-evolved riboswitches and computationally redesigned transcription factors. Thus, powered by the rapidly expanding repertoire of biosensors and streamlined processes for automated genetic circuit design, a greater variety of complex selection circuits can be built, with resulting impacts on drug discovery and industrial biotechnology.

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Citations

Jul 19, 2019·Expert Opinion on Drug Discovery·Yu DuBin Hong
May 12, 2020·Frontiers in Bioengineering and Biotechnology·Hyewon LeeSeung-Goo Lee
Dec 1, 2018·Nature Communications·Soo-Jin YeomSeung-Goo Lee
Jun 26, 2020·Frontiers in Microbiology·Carolyne Caetano GonçalvesNádia Skorupa Parachin
Dec 1, 2020·Critical Reviews in Food Science and Nutrition·Prasoon KumarPratyoosh Shukla
Aug 23, 2019·Biotechnology Advances·Luísa Czamanski NoraRafael Silva-Rocha
May 11, 2021·Frontiers in Microbiology·Nicholas J ReichartRoland Hatzenpichler
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Sep 27, 2019·Environmental Science & Technology·Line Roager, Eva C Sonnenschein
Dec 22, 2021·Microbial Biotechnology·Víctor de Lorenzo

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Methods Mentioned

BETA
FACS
biosensor
fluorescence-activated cell sorting
biosensors
PCR
fluorescence-assisted cell sorting

Software Mentioned

Verilog
Rosetta
SIGEX
IMPLY
METREX
Cello
FACS

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