PMID: 9168300Apr 1, 1997Paper

The ex vivo expansion capacity of normal human bone marrow cells is dependent on experimental conditions: role of the cell concentration, serum and CD34+ cell selection in stroma-free cultures

Hematology and Cell Therapy
A PoloniL Douay

Abstract

The present study was conducted to establish defined culture conditions for ex vivo expansion of normal human bone marrow cells. We investigated the role of three experimental expansion parameters: the cell concentration in the initial culture medium, the role of animal serum, human plasma and serum-free substitute, and the expansion potential of mononucleated cells (MNC) versus CD34+ cells. Cells were cultured in suspension with stem cell factor (SCF), IL3, IL6 and Erythropoietin (Epo) for 10 days. 1) Reducing the cell concentration from 3 x 10(4) to 1.5 x 10(3)/ml increased total cell expansion almost 20 fold, progenitor expansion more than 3 fold, and the maintenance of long term culture-initiating cells (LTC-IC). 2) In medium containing a serum-free substitute, total and CD34+ cell expansion was 3 times greater than in medium containing 1-10% human AB plasma or 25% animal serum. 3) The expansion potential of selected CD34+ cells was significantly greater than that of the total MNC population. However, taking into account the cell loss due to CD34+ selection, the overall results for quantitative expansion in relation to the initial number of MNCS favor the use of non-selected MNCS. 4) SCF + IL3 + IL6 was clearly the best com...Continue Reading

Citations

Jan 22, 2004·Archives of Medical Research·Héctor MayaniPatricia Flores-Guzmán
Feb 10, 2000·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·L Douay
Aug 10, 2000·British Journal of Haematology·D Y ZangF Appelbaum
May 22, 2001·Journal of Hematotherapy & Stem Cell Research·L KobariL Douay
Jul 17, 2001·Journal of Hematotherapy & Stem Cell Research·L Douay
Aug 20, 2002·Journal of Hematotherapy & Stem Cell Research·Jean-Marc BerthoDominique Thierry
Nov 11, 1999·British Journal of Haematology·P D Emanuel
Jun 6, 2006·Laboratory Hematology : Official Publication of the International Society for Laboratory Hematology·Azza A MohamedRaafat M Riad
Jul 17, 2004·Journal of Cellular and Molecular Medicine·Mihaela ChivuC Cernescu
Dec 7, 2011·Transfusion·Jo-Anna Reems
Feb 28, 2002·Molecular Therapy : the Journal of the American Society of Gene Therapy·Kimberley A KlugeCynthia E Dunbar

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