The ex vivo isolated skeletal microvessel preparation for investigation of vascular reactivity.

Journal of Visualized Experiments : JoVE
Joshua T ButcherJefferson C Frisbee

Abstract

The isolated microvessel preparation is an ex vivo preparation that allows for examination of the different contributions of factors that control vessel diameter, and thus, perfusion resistance(1-5). This is a classic experimental preparation that was, in large measure, initially described by Uchida et al.(15) several decades ago. This initial description provided the basis for the techniques that was extensively modified and enhanced, primarily in the laboratory of Dr. Brian Duling at the University of Virginia(6-8), and we present a current approach in the following pages. This preparation will specifically refer to the gracilis arteriole in a rat as the microvessel of choice, but the basic preparation can readily be applied to vessels isolated from nearly any other tissue or organ across species(9-13). Mechanical (i.e., dimensional) changes in the isolated microvessels can easily be evaluated in response to a broad array of physiological (e.g., hypoxia, intravascular pressure, or shear) or pharmacological challenges, and can provide insight into mechanistic elements comprising integrated responses in an intact, although ex vivo, tissue. The significance of this method is that it allows for facile manipulatio...Continue Reading

Citations

Jun 23, 2018·American Journal of Physiology. Heart and Circulatory Physiology·Kent A LemasterJefferson C Frisbee
Aug 16, 2014·Journal of Applied Physiology·Shyla C StanleyJefferson C Frisbee
May 16, 2019·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Brayden D HalvorsonJefferson C Frisbee
Jul 15, 2021·Analytical and Bioanalytical Chemistry·Harmain Rafi, Alexander G Zestos
Jun 26, 2021·American Journal of Physiology. Heart and Circulatory Physiology·E N BurrageI M Olfert

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