The exchange of Arg-Gly-Asp (RGD) and Arg-Tyr-Asp (RYD) binding sequences in a recombinant murine Fab fragment specific for the integrin alpha IIb beta 3 does not alter integrin recognition.

The Journal of Biological Chemistry
T J KunickiD S Annis

Abstract

The murine monoclonal antibody OPG2 is an excellent paradigm of natural RGD ligands and binds specifically to alpha IIb beta 3 integrin. A reactive Arg103-Tyr104-Asp105 (RYD) tripeptide is located in an extended loop, the third complementarity-determining region of the heavy chain (H3). When compared to other RGD ligands, the RYD tripeptide of OPG2 is unique, in that the side chains are fixed in a stable orientation that we have defined by x-ray crystallography. In this study, we express OPG2 H chain segments (Fd) and kappa chains as components of active, Fab heterodimers by coinfection of Spodoptera frugiperda cell lines with recombinant baculoviruses containing cDNA specific for each protein. Recombinant AP7 Fd segments are generated from the parent OPG2 Fd segments by replacement of Tyr104 with Gly, while recombinant AP7E Fd segments are produced from AP7 Fd segments, by exchange of Asp105 with Glu. Neither the free Fd segments nor the free kappa chains of OPG2 or AP7 can bind to alpha IIb beta 3. The AP7 Fab fragment, like the parent OPG2 Fab, binds strongly to purified alpha IIb beta 3 but weakly, if at all, to purified alpha V beta 3. The affinity of OPG2 and AP7 Fab fragments for gel-filtered platelets, whether nonstimul...Continue Reading

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Citations

Aug 5, 1998·Toxicon : Official Journal of the International Society on Toxinology·H H Chang, S J Lo
Dec 22, 1999·Clinical and Experimental Immunology·Y J Deng, A L Notkins
Dec 1, 1996·Molecular Biology of the Cell·F Díaz-GonzálezM H Ginsberg
Mar 5, 1999·Critical Reviews in Toxicology·R HatiK K Bhattacharyya
Feb 14, 1997·The Journal of Biological Chemistry·T J KunickiB Felding-Habermann
Aug 23, 1996·The Journal of Biological Chemistry·T J KunickiS J Shattil
Feb 13, 2004·The Journal of Biological Chemistry·Paul H WeinrebShelia M Violette
Oct 29, 1998·Biochemical and Biophysical Research Communications·R KodandapaniK R Ely

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