The experimental research of R-phycoerythrin subunits on cancer treatment: a new photosensitizer in PDT

Cancer Biotherapy & Radiopharmaceuticals
Bei HuangZhen-gang Li

Abstract

The mouse tumor cell S180 and human liver carcinoma cell SMC 7721 cells were first treated with R-PE and its subunits (alpha, beta, gamma subunits), then irradiated with Argon laser (496 nm, 28.8 J/cm2). Survival rate was measured by MTT method. In order to compare the phototoxicity in normal cells, the mouse marrow cells were treated with photofrin II and beta-subunit, irradiated with 45 J/cm2 of light; survival rate was also measured by MTT method. The result showed that R-PE subunits had better PDT effect on s180 cells than R-PE and lower phototoxicity in marrow cells than photofrin II. Flow cytometric analysis showed that PDT results in a growth inhibition and a G0-G1 cell cycle arrest in SMC 7721 cells. The tumor cells inhibited by PDT in vivo were morphologically observed by TEM, the tumor cell death was due to the occlusion of tumor blood vessels and inducement of cell programmed death in nuclei. Therefore, with the advantage in special fluorescence activity, low molecular weight, good light absorbent character and weak phototoxicity, R-PE subunit is an attractive option for improving the selectivity of PDT.

References

Jan 1, 1995·Lasers in Surgery and Medicine·A M FisherC J Gomer
Oct 1, 1993·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·K FuruseC Konaka
Mar 17, 1993·Journal of the National Cancer Institute·H I Pass
Jan 15, 1997·Journal of the National Cancer Institute·T Reynolds
Jan 1, 1997·Lasers in Surgery and Medicine·I K Koehler
Feb 1, 1997·Archives of Otolaryngology--head & Neck Surgery·J F SavaryH van den Bergh
Aug 1, 1997·Photochemistry and Photobiology·A M FisherC J Gomer
Apr 16, 1998·Journal of Photochemistry and Photobiology. B, Biology·D Kessel, Y Luo
Jun 24, 1998·Journal of the National Cancer Institute·T J DoughertyQ Peng
Aug 30, 2000·International Journal of Cancer. Journal International Du Cancer·M B VrouenraetsG A van Dongen

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Citations

Jul 29, 2010·Photomedicine and Laser Surgery·Aline Helena Araujo MachadoNewton Soares da Silva
May 10, 2003·Journal of Clinical Laser Medicine & Surgery
Mar 30, 2006·Protein Expression and Purification·Jian-Feng NiuCheng-Kui Tseng
Feb 13, 2015·Critical Reviews in Food Science and Nutrition·Hasina BegumMohamed Shariff
Mar 17, 2016·World Journal of Biological Chemistry·Ravi Raghav SonaniDatta Madamwar
Apr 17, 2008·Lasers in Surgery and Medicine·Rainer J EgliMichael Leunig
May 31, 2019·Marine Drugs·Maria Dolores TorresHerminia Domínguez
Oct 14, 2019·Bioprocess and Biosystems Engineering·Yuanchao XuLu Lin
Jul 10, 2020·The Journal of Physical Chemistry Letters·Pankaj BharmoriaJoão A P Coutinho
Dec 21, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jian-Feng NiuBai-Cheng Zhou
Aug 8, 2012·Journal of Agricultural and Food Chemistry·Raúl E CianOlga Martínez-Augustin

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