PMID: 30024694Mar 1, 2016Paper

The experimental study of the effect of ASCs on the skin expansion rate in rabbit

Zhonghua zheng xing wai ke za zhi = Zhonghua zhengxing waike zazhi = Chinese journal of plastic surgery
Zemin ZhangBiaobing Yang

Abstract

To explore the effect of adipose-derived stem cells (ASCs) on the skin expansion rate in rabbit. The rabbit ASCs were isolated from fat tissue and cultured in vitro. The ADSCs were identified by cell immunofluorescence and marked by Edu staining.20 new Zealand rabbits were randomly divided into experimental(n =10) and control group(n =10).An area of 1.5 cm ×1.5 cm on the one side back of each rabbit was tattooed and one 30 ml round expander was implanted subcutaneously. ASCs suspension (1 ml) was injected subcutaneously in the experimental group, while serum free DMEM medium(1 ml) in control group. The expansion was proceeded regularly under constant pressure for 4 weeks.The expanded tattooed square area was measured on the 7th,14th,28th day and analyzed statistically. The expanded skin was harvested for histological study. Immunohistochemical staining was used to detect the expression of vascular endothelial cell marker CD31,and the microvessel density determination. The expression of epidermal growth factor (EGF) and vascular endothelial growth factor(VEGF)was detected by ELISA for skin tissue specificity. Western Blot was used for detection of CK19 in the epidermal cells. The expanded skin thickness and expansion rate in exp...Continue Reading

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Related Papers

Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery
Yongming YaoBiaobing Yang
Zhonghua shao shang za zhi = Zhonghua shaoshang zazhi = Chinese journal of burns
Y M YaoB B Yang
© 2022 Meta ULC. All rights reserved