The expression and action of granulocyte macrophage-colony stimulating factor and its interaction with TGF-beta in endometrial carcinoma

Gynecologic Oncology
D RipleyN Chegini

Abstract

Previous studies have demonstrated that normal human endometrium expresses granulocyte macrophage-colony stimulating factor (GM-CSF) and GM-CSF receptors. Because GM-CSF is administer to cancer patients following chemotherapy, GM-CSF may directly or through interaction with ovarian steroids and other cytokines alter the behavior of endometrial cancer. The aim of this study was to determine the expression of GM-CSF and receptors in endometrial carcinoma and its direct effect and interaction with transforming growth factor beta (TGF-beta) on Ishikawa cells, a human endometrial carcinoma cell line. GM-CSF, GM-CSF receptors, TGF-beta1, and TGF-beta type II receptor expression were evaluated using quantitative reverse transcription polymerase chain reaction (Q-RT-PCR). The effect of GM-CSF on DNA synthesis, cell proliferation, expression of GM-CSF, TGF-beta1, and TGF-beta receptor, and their regulation by ovarian steroids was determined by the rate of [(3)H]thymidine incorporation, MTT assay, Q-RT-PCR, and ELISA, respectively. Endometrial carcinomas express significantly higher GM-CSF and GM-CSF alpha and beta receptor mRNA compared with normal postmenopausal endometrium. GM-CSF at various doses had no significant effect on the rate...Continue Reading

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Citations

Dec 18, 2008·Reproductive Sciences·Tannaz ToloubeydokhtiNasser Chegini
Oct 10, 2003·Cancer Investigation·Dezhong Joshua LiaoFazlul H Sarkar
Dec 23, 2017·Cell Communication and Signaling : CCS·Wu FeiMakoto Saegusa
Jun 10, 2005·International Journal of Cancer. Journal International Du Cancer·Joyce C L LeoValerie C L Lin
Feb 21, 2004·The Journal of Biological Chemistry·Ligong ChenAnne Woods
Nov 8, 2020·Stem Cell Research & Therapy·Leonardo MartinsSang Won Han

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