The expression and activity of β-catenin in the thalamus and its projections to the cerebral cortex in the mouse embryo.

BMC Neuroscience
Thomas PrattDavid J Price

Abstract

The mammalian thalamus relays sensory information from the periphery to the cerebral cortex for cognitive processing via the thalamocortical tract. The thalamocortical tract forms during embryonic development controlled by mechanisms that are not fully understood. β-catenin is a nuclear and cytosolic protein that transduces signals from secreted signaling molecules to regulate both cell motility via the cytoskeleton and gene expression in the nucleus. In this study we tested whether β-catenin is likely to play a role in thalamocortical connectivity by examining its expression and activity in developing thalamic neurons and their axons. At embryonic day (E)15.5, the time when thalamocortical axonal projections are forming, we found that the thalamus is a site of particularly high β-catenin mRNA and protein expression. As well as being expressed at high levels in thalamic cell bodies, β-catenin protein is enriched in the axons and growth cones of thalamic axons and its growth cone concentration is sensitive to Netrin-1. Using mice carrying the β-catenin reporter BAT-gal we find high levels of reporter activity in the thalamus. Further, Netrin-1 induces BAT-gal reporter expression and upregulates levels of endogenous transcripts e...Continue Reading

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Citations

Mar 29, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Anne Marion TaylorErin M Schuman
Oct 11, 2016·Current Genetic Medicine Reports·Lulu I T KorsakMichael R Akins
Jul 16, 2013·Developmental Neurobiology·Marianna CrispinoAntonio Giuditta
Nov 9, 2017·Developmental Neurobiology·Christopher J Costa, Dianna E Willis

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Methods Mentioned

BETA
transgenic
PCR
transfection
myristoylation

Software Mentioned

ImageJ
PerlPrimer
Opticon software
LAGAN
BLAST
Sigmaplot
ENSEMBL

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