The expression and significance of CATSPER1 in human testis and ejaculated spermatozoa

Asian Journal of Andrology
Hong-Gang LiCheng-Liang Xiong

Abstract

To investigate the distribution of cation channel of sperm 1 (CATSPER1) protein and the presence of CATSPER1 mRNA in human testis and ejaculated spermatozoa. The influence of anti-human CATSPER1 antibody upon human sperm motility was used to evaluate the function of human CATSPER1 and to estimate its possible use as a target for immunocontraception. Human ejaculated sperm from normozoospermic donors (n = 12) and liquid nitrogen frozen human testis were used for the study of mRNA and protein expression of CATSPER1 by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry, respectively. Spermatozoa from normozoospermic donors (n = 12) were individually processed using a swim-up procedure and were then incubated with CATSPER1 antibody at final concentrations of 20, 4 and 0.8 microg/mL. After 1, 2 and 6 h incubation, progressive motility and fast progressive motility were measured by means of computer-assisted semen analysis. CATSPER1 transcript was detected in both human testis and each human ejaculated semen sample. CATSPER1 protein expressed in the membrane of spermatid and was localized in the principal piece of the sperm tail. The application of CATSPER1 antibody at all concentrations significantly i...Continue Reading

References

Oct 12, 2001·Nature·D L Garbers
Oct 25, 2001·Proceedings of the National Academy of Sciences of the United States of America·T A QuillD L Garbers
Sep 21, 2002·Lancet·G Charles OstermeierStephen A Krawetz
Oct 3, 2003·Proceedings of the National Academy of Sciences of the United States of America·Ondrej Podlaha, Jianzhi Zhang
Dec 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Anne E CarlsonDonner F Babcock
May 18, 2005·Asian Journal of Andrology·Rui Pires Martins, Stephen A Krawetz
Jun 3, 2005·Molecular Biology and Evolution·Ondrej PodlahaJianzhi Zhang

❮ Previous
Next ❯

Citations

Oct 18, 2011·Asian Journal of Andrology·Zhen ZhangFei Zhou
Jan 15, 2013·Molecular Human Reproduction·Minerva Mata-RochaNorma Oviedo
Aug 23, 2007·Asian Journal of Andrology·Elena MorettiGiulia Collodel
Mar 10, 2015·The Tohoku Journal of Experimental Medicine·Houyang ChenYuxin Tang
May 28, 2014·American Journal of Reproductive Immunology : AJRI·Mónica H Vazquez-LevinCarolina Veaute
Sep 12, 2015·Journal of Assisted Reproduction and Genetics·Fangpeng ShuXiangming Mao
Apr 24, 2010·Molecular and Cellular Endocrinology·E A McLaughlin, R J Aitken
Oct 31, 2013·Expert Review of Proteomics·Karolina Nowicka-Bauer, Maciej Kurpisz
Nov 4, 2020·Molecular Reproduction and Development·Jasmer DalalPradeep Kumar

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

American Journal of Human Genetics
Matthew R AvenariusRichard J H Smith
Proceedings of the National Academy of Sciences of the United States of America
T A QuillD L Garbers
Proceedings of the National Academy of Sciences of the United States of America
Anne E CarlsonDonner F Babcock
Proceedings of the National Academy of Sciences of the United States of America
Huayu QiD E Clapham
© 2022 Meta ULC. All rights reserved