Abstract
To investigate the expression and significance of mechanistic target of rapamycin complex 1(mTORC1) in diabetic retinopathy (DR), and to find new targets and new methods for the treatment of DR. A DR rat model was prepared by general feeding combined with intraperitoneal injection of 10% streptozotocin (60 mg/kg). The rats were randomly divided into a control group (NDM group) and a diabetes group (DM group). Three months later, the degrees of retinopathy was determined using hematoxylin and eosin staining, and the levels of p-S6, VEGF, and PEDF proteins were detected by immunohistochemistry and western blotting. Human retinal capillary endothelial cells (HRCECs) were cultured in high glucose (HG) conditions, then treated with rapamycin or transfected with siTSC1.The protein levels of p-S6 were assessed by western blotting. The 5-ethynyl-2'-deoxyuridine assay was used to detect cell proliferation, and the Transwell assay was used to detect cell migration. A DM rat model was successfully developed. The expressions of p-S6 and VEGF proteins were significantly increased in the DM group (p < 0.05), and the expression of PEDF protein was significantly decreased compared with the NDM group (p < 0.05). In vitro, the p-S6 protein, as w...Continue Reading
References
Aug 23, 1991·Science·J HeitmanM N Hall
Dec 16, 2010·Nature Reviews. Molecular Cell Biology·Roberto ZoncuDavid M Sabatini
Jan 10, 2012·Journal of Diabetes and Its Complications·Mohammad Shamsul OlaAhmed M Abu El-Asrar
Jun 26, 2013·Journal of Visualized Experiments : JoVE·Jonathan C ChouAmani A Fawzi
Apr 18, 2014·Diabetes·Patrice E FortThomas W Gardner
Jun 16, 2015·Survey of Ophthalmology·Tapan BehlAnita Kotwani
Jun 21, 2015·Experimental Eye Research·Elizabeth A StewartWinfried M Amoaku
Jun 24, 2015·Biochemical and Biophysical Research Communications·Huanqi ShiXiaomei Zhang
Oct 3, 2015·Advances in Experimental Medicine and Biology·Melissa A Calton, Douglas Vollrath
Mar 22, 2016·Current Neuropharmacology·Michelino Di RosaLucia Malaguarnera
Mar 29, 2016·The Journal of Clinical Investigation·Dianxin LiuSheila Collins
Jun 16, 2016·Cell Metabolism·Brian K Kennedy, Dudley W Lamming
Jul 31, 2016·Diabetes·Jose-Andres C PortilloCarlos S Subauste
Mar 14, 2017·Oxidative Medicine and Cellular Longevity·Andréia Cristina de MeloÁlvaro Henrique Ingles Garces
Mar 16, 2017·Investigative Ophthalmology & Visual Science·Pei ZhuangShunbin Xu
Apr 16, 2017·Current Opinion in Cell Biology·Issam Ben-Sahra, Brendan D Manning
Apr 20, 2017·Ophthalmologica. Journal international d'ophtalmologie. International journal of ophthalmology. Zeitschrift für Augenheilkunde·Ursula Schmidt-ErfurthAnat Loewenstein
Dec 14, 2017·Nature·Haijuan YangNikola P Pavletich
Apr 6, 2018·Experimental Cell Research·Lin ZhuXianghong Zhang
Jan 18, 2019·Experimental and Therapeutic Medicine·Qian LuXiao-Xing Yin
Feb 26, 2019·Cell Death & Disease·Siran XuYongzhang Luo
Mar 25, 2019·Cell Communication and Signaling : CCS·Ting LuZheng-Hong Qin
Apr 27, 2019·Journal of Experimental & Clinical Cancer Research : CR·Ting WangLi Cai
Apr 27, 2019·The Journal of Physiology·Aurélie DocquierHenri Bernardi
Sep 11, 2019·Nucleic Acids Research·Jae-Woong ChangJeongsik Yong
Sep 19, 2019·The Journal of Clinical Investigation·Kaushal AsraniTamara L Lotan