The expression and significance of mTORC1 in diabetic retinopathy

BMC Ophthalmology
Yanli LiuMeixia An

Abstract

To investigate the expression and significance of mechanistic target of rapamycin complex 1(mTORC1) in diabetic retinopathy (DR), and to find new targets and new methods for the treatment of DR. A DR rat model was prepared by general feeding combined with intraperitoneal injection of 10% streptozotocin (60 mg/kg). The rats were randomly divided into a control group (NDM group) and a diabetes group (DM group). Three months later, the degrees of retinopathy was determined using hematoxylin and eosin staining, and the levels of p-S6, VEGF, and PEDF proteins were detected by immunohistochemistry and western blotting. Human retinal capillary endothelial cells (HRCECs) were cultured in high glucose (HG) conditions, then treated with rapamycin or transfected with siTSC1.The protein levels of p-S6 were assessed by western blotting. The 5-ethynyl-2'-deoxyuridine assay was used to detect cell proliferation, and the Transwell assay was used to detect cell migration. A DM rat model was successfully developed. The expressions of p-S6 and VEGF proteins were significantly increased in the DM group (p < 0.05), and the expression of PEDF protein was significantly decreased compared with the NDM group (p < 0.05). In vitro, the p-S6 protein, as w...Continue Reading

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Citations

Jul 3, 2021·International Journal of Molecular Sciences·Petra Grubić RotkvićLuka Rotkvić
Aug 8, 2021·International Journal of Molecular Sciences·Vadim V KlimontovAnton I Korbut

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Methods Mentioned

BETA
dissection
transfection

Software Mentioned

SPSS

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