The expression of a bean PGIP in transgenic wheat confers increased resistance to the fungal pathogen Bipolaris sorokiniana

Molecular Plant-microbe Interactions : MPMI
Michela JanniRenato D'Ovidio

Abstract

A possible strategy to control plant pathogens is the improvement of natural plant defense mechanisms against the tools that pathogens commonly use to penetrate and colonize the host tissue. One of these mechanisms is represented by the host plant's ability to inhibit the pathogen's capacity to degrade plant cell wall polysaccharides. Polygalacturonase-inhibiting proteins (PGIP) are plant defense cell wall glycoproteins that inhibit the activity of fungal endopolygalacturonases (endo-PGs). To assess the effectiveness of these proteins in protecting wheat from fungal pathogens, we produced a number of transgenic wheat lines expressing a bean PGIP (PvPGIP2) having a wide spectrum of specificities against fungal PGs. Three independent transgenic lines were characterized in detail, including determination of the levels of PvPGIP2 accumulation and its subcellular localization and inhibitory activity. Results show that the transgene-encoded protein is correctly secreted into the apoplast, maintains its characteristic recognition specificities, and endows the transgenic wheat with new PG recognition capabilities. As a consequence, transgenic wheat tissue showed increased resistance to digestion by the PG of Fusarium moniliforme. These...Continue Reading

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Citations

Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Vincenzo LionettiFelice Cervone
Aug 16, 2013·Molecular Plant-microbe Interactions : MPMI·Ilaria MoscettiRenato D'Ovidio
Aug 7, 2010·Annual Review of Phytopathology·David B CollingeMichael F Lyngkjaer
Feb 25, 2009·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Anna FarinaRenato D'Ovidio
Jan 13, 2011·The Plant Journal : for Cell and Molecular Biology·Monica De CaroliGabriella Piro
Dec 10, 2014·Functional & Integrative Genomics·Aiyun WangZengyan Zhang
Aug 15, 2014·Molecular Genetics and Genomics : MGG·Laura GazzaNorberto E Pogna
Dec 10, 2014·Plant Molecular Biology·Rui WangYongjun Lin
Jul 2, 2016·Molecular Plant-microbe Interactions : MPMI·Silvio TundoRenato D'Ovidio
Nov 5, 2016·Molecular Plant-microbe Interactions : MPMI·Silvio TundoRenato D'Ovidio
Nov 5, 2016·Plant Biotechnology Journal·Udaykumar KageCurt McCartney
Nov 14, 2018·Molecular Plant-microbe Interactions : MPMI·Giulia MandalàRenato D'Ovidio
Jun 5, 2019·Hereditas·Eduardo VenskeAntonio Costa de Oliveira

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