The Expression of a Novel Mitochondrially-Encoded Gene in Gonadic Precursors May Drive Paternal Inheritance of Mitochondria

PloS One
Liliana MilaniMarco Passamonti

Abstract

Mitochondria have an active role in germ line development, and their inheritance dynamics are relevant to this process. Recently, a novel protein (RPHM21) was shown to be encoded in sperm by the male-transmitted mtDNA of Ruditapes philippinarum, a species with Doubly Uniparental Inheritance (DUI) of mitochondria. In silico analyses suggested a viral origin of RPHM21, and we hypothesized that the endogenization of a viral element provided sperm mitochondria of R. philippinarum with the ability to invade male germ line, thus being transmitted to the progeny. In this work we investigated the dynamics of germ line development in relation to mitochondrial transcription and expression patterns using qPCR and specific antibodies targeting the germ line marker VASPH (R. philippinarum VASA homolog), and RPHM21. Based on the experimental results we conclude that both targets are localized in the primordial germ cells (PGCs) of males, but while VASPH is detected in all PGCs, RPHM21 appears to be expressed only in a subpopulation of them. Since it has been predicted that RPHM21 might have a role in cell proliferation and migration, we here suggest that PGCs expressing it might gain advantage over others and undertake spermatogenesis, accou...Continue Reading

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Citations

Apr 8, 2017·Histochemistry and Cell Biology·Liliana MilaniMaria Gabriella Maurizii
Apr 27, 2017·Molecular Biology and Evolution·Andrea PozziMarco Passamonti
Sep 2, 2017·The Journal of Experimental Biology·Patricia CavelierClaude Delsert
Apr 16, 2016·PloS One·Stefano BettinazziMarco Passamonti
Jun 24, 2016·Scientific Reports·Ming WenYunhan Hong
Jan 18, 2018·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Fabrizio GhiselliLiliana Milani
May 24, 2019·Integrative and Comparative Biology·Fabrizio GhiselliLiliana Milani
Dec 4, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Liliana Milani, Fabrizio Ghiselli
Sep 11, 2019·Integrative and Comparative Biology·Justin C HavirdGeoffrey E Hill
Sep 7, 2017·Histochemistry and Cell Biology·Liliana MilaniMaria Gabriella Maurizii
Dec 1, 2016·Current Zoology·Liliana MilaniMarco Passamonti
Feb 3, 2018·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Elisabetta PunziMarco Passamonti
May 5, 2021·Integrative and Comparative Biology·Fabrizio GhiselliLiliana Milani
Dec 5, 2020·Biochimica Et Biophysica Acta. Molecular Cell Research·Karla Pacheco de Melo, Mariana Camargo

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Methods Mentioned

BETA
ELISA
Electrophoresis

Software Mentioned

Primer3
Mega
TASSER
Chimera
Leica
vasph

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