The expression of aquaporin-1 in the medulla of the kidney is dependent on the transcription factor associated with hypertonicity, TonEBP.

The Journal of Biological Chemistry
Miguel A LanaspaTomas Berl

Abstract

Expression of aquaporin-1 (AQP1) and -2 (AQP2) channels in the kidney are critical for the maintenance of water homeostasis and the operation of the urinary concentrating mechanism. Hypertonic stress induced in inner medullary (IMCD3) cells by addition of NaCl to the medium substantially up-regulated the mRNA and protein expression of AQP1, suggesting that its activation occurs at a transcriptional and a translational levels. In contrast, no up-regulation of AQP1 was observed when these cells were exposed to the same tonicity by addition of urea. To explore the transcriptional activation of aqp1 under hypertonic stress, we examined the role of the transcription factor associated with hypertonicity, TonEBP. Treatment of IMCD3 cells with the TonEBP inhibitor rottlerin or silencing its expression with specific shRNA technology led to a substantial reduction in AQP1 expression under hypertonic conditions. Moreover, we defined a conserved TonEBP binding site located 811 bp upstream of the aqp1 exon that is essential for its expression. Single site-directed mutation of this TonE site led to a 54 ± 5% (p < 0.01) decrease in AQP1 luciferase-driven activity under hypertonic stress. TonEBP mutant mice display marked decrement in the expr...Continue Reading

References

Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S NielsenH W Harris
Jun 1, 1996·Kidney International·J S Handler, H M Kwon
Jan 1, 1997·Journal of the American Society of Nephrology : JASN·A B MaunsbachS Nielsen
Jan 1, 1997·Annual Review of Physiology·M B BurgD Kültz
May 20, 1998·Biochemical and Biophysical Research Communications·W JenqG Ramirez
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·J SchnermannA S Verkman
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·H MiyakawaH M Kwon
Jun 3, 2000·American Journal of Physiology. Renal Physiology·S K WooH M Kwon
Sep 21, 2000·The Journal of Biological Chemistry·Y NakayamaS M Bagnasco
Jul 21, 2001·The New England Journal of Medicine·L S KingP Agre
Jul 28, 2001·Kidney International·J S Handler, H M Kwon
Oct 25, 2001·Journal of the American Society of Nephrology : JASN·Jung H ChaH Moo Kwon
Jan 10, 2002·Nature Structural Biology·James C StroudLin Chen
Mar 7, 2002·American Journal of Physiology. Renal Physiology·Hongyu ZhaoDavid M Cohen
Apr 2, 2002·Biochemical and Biophysical Research Communications·Fuminori Umenishi, Robert W Schrier
Jul 26, 2002·Molecular and Cellular Biology·Seung Kyoon WooH Moo Kwon
Oct 22, 2002·American Journal of Physiology. Renal Physiology·R StormK Maric
May 24, 2003·Annals of the New York Academy of Sciences·Juan M CapassoTomas Berl
Feb 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Cristina López-RodríguezEric N Olson
Jul 1, 2004·American Journal of Physiology. Renal Physiology·Ki-Hwan HanH Moo Kwon
Jul 13, 2004·Proceedings of the National Academy of Sciences of the United States of America·William Y GoSteffan N Ho
Jan 14, 2005·Journal of the American Society of Nephrology : JASN·Fuminori UmenishiRobert W Schrier
Jan 27, 2005·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Keizo KasonoSan-e Ishikawa
Nov 18, 2005·Biochemical and Biophysical Research Communications·Fuminori UmenishiRobert W Schrier
Apr 28, 2006·Journal of the American Society of Nephrology : JASN·Udo HaslerPierre-Yves Martin
Aug 24, 2006·American Journal of Physiology. Renal Physiology·Miguel A LanaspaVíctor Sorribas
Oct 4, 2006·Proceedings of the National Academy of Sciences of the United States of America·Morgan GallazziniMaurice B Burg
Oct 27, 2006·Contributions to Nephrology·Joan D Ferraris, Maurice B Burg
Aug 11, 2007·Proceedings of the National Academy of Sciences of the United States of America·Miguel A LanaspaTomas Berl
Aug 19, 2007·Trends in Pharmacological Sciences·Stephen P Soltoff
Jan 14, 2009·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Sachin GajghateMakarand V Risbud

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Jan 28, 2012·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Wenmin JinLijun Yao
Mar 5, 2014·Journal of Physiology and Biochemistry·Silvana L Della PennaBelisario E Fernández
Oct 26, 2013·Basic Research in Cardiology·Arkady RutkovskiyJarle Vaage
Apr 27, 2013·Journal of Molecular Signaling·Chris Yk Cheung, Ben Cb Ko
Aug 25, 2015·Biochemical and Biophysical Research Communications·Kai Guo, Faguang Jin
Dec 3, 2014·European Journal of Obstetrics, Gynecology, and Reproductive Biology·G T LeeJ A Arroyo
Mar 7, 2018·Proceedings of the National Academy of Sciences of the United States of America·Miguel A LanaspaRichard J Johnson
Mar 21, 2018·BMC Molecular Biology·Yihui ZhaiJiaojiao Liu
Jan 10, 2019·American Journal of Physiology. Renal Physiology·Jayalakshmi LakshmipathiDonald E Kohan
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Nov 1, 2018·Journal of Cellular Physiology·Shadia BeainiNassim Fares
May 9, 2019·American Journal of Physiology. Cell Physiology·Yair SermanCarlos E Irarrázabal
Jul 6, 2014·American Journal of Physiology. Renal Physiology·Xiaoming ZhouNaomi E Aronson
Sep 17, 2017·Oncotarget·Daniel Peña-OyarzunAlfredo Criollo
Jan 1, 2015·American Journal of Physiology. Renal Physiology·Ivonne Loeffler, Gunter Wolf
Nov 14, 2014·American Journal of Physiology. Renal Physiology·Hong WangXiaoming Zhou
Aug 5, 2020·Proceedings of the National Academy of Sciences of the United States of America·Raj KumarJoan D Ferraris
Feb 15, 2017·Nature Communications·Ana Andres-HernandoMiguel A Lanaspa
Oct 6, 2020·Journal of Diabetes Research·Lusha CenYi Cao
Jan 21, 2020·Heliyon·Erika A SeyahianElsa Zotta
Dec 23, 2017·The American Journal of Pathology·Mandakini PatelJeffrey L Barnes
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