PMID: 20646666Jul 22, 2010Paper

The expression of catechol O-methyltransferase gene in colorectal cancer

Zhonghua wai ke za zhi [Chinese journal of surgery]
Wen-ming WuTai-ping Zhang

Abstract

To study the expression of the gene and coding protein of catechol o-methyltransferase (COMT) in the colorectal cancer and distant normal mucosa. The tumor tissues and distant normal mucosa of 22 patients with colorectal cancer received surgical treatment from January to August 2009 were collected. Total RNA and protein were extracted and tested using reverse transcription polymerase chain reaction (RT-PCR), Western blot was used to detect the expression of COMT in the tumor tissue and normal mucosa. Tissue array was performed to verify the COMT protein expression. The gray scale scan was used to differentiate the COMT expression level of the mRNA and protein between the two groups. The tested mean light density of COMT mRNA was 53 514 +/- 15 513 in the tumor tissue group and 4529 +/- 1698 in the normal mucosa group, the expression of COMT gene in colorectal cancer tissue was significantly higher than that in the normal mucosa (P < 0.05). Western blot results showed that the expression level of soluble COMT protein in the colorectal cancer tissue was significantly higher than that in the corresponding distant normal mucosa (mean light density 54 967 +/- 11 919 vs. 25 962 +/- 6713) (P < 0.05), but the expression of membrane-boun...Continue Reading

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