PMID: 6662113Dec 15, 1983Paper

The expression of different monooxygenases supported by cytochrome P-450 in neonatal rats and in primary fetal hepatocytes in culture

European Journal of Biochemistry
P KremersJ E Gielen

Abstract

In rat liver, the perinatal development of various monooxygenase activities follows different patterns, depending upon the reaction studied. The ontogeny of the 6 beta-, 7 alpha- and 16 alpha-testosterone hydroxylase activities differs very significantly. Aldrin epoxidase and steroid-metabolizing monooxygenases are expressed in primary fetal rat liver cells in culture after treatment in vitro with dexamethasone. Testosterone is not metabolized by the control cells and is hydroxylated on the 6 beta and 16 alpha positions following the addition of corticoids to the culture medium. The dose and time curves vary according to the hydroxylated position of the steroid. Aldrin epoxidase activity is nearly undetectable in the control cells, but is present and is inducible by phenobarbital following treatment with the corticoid. Phenobarbital induces aldrin epoxidase in the absence of dexamethasone in the culture medium, providing that the cells are pretreated with the corticoid for 48 h. The use of antibodies against the main cytochrome P-450 species purified from adult and phenobarbital-treated rats confirms that a similar cytochrome P-450 can be induced in fetal cells in culture. The perinatal regulation of biological events, such as ...Continue Reading

References

Jan 1, 1979·Archives of Biochemistry and Biophysics·W E FahlH C Pitot
May 1, 1977·Journal of Steroid Biochemistry·J A GustafssonA Ahlén
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Aug 1, 1983·Biochemical Society Transactions·P Kremers, J E Gielen
Apr 28, 1981·Biochemistry·F P GuengerichM B Mitchell
May 1, 1981·European Journal of Biochemistry·P KremersJ E Gielen
Sep 1, 1981·European Journal of Biochemistry·P KremersJ E Gielen
May 30, 1980·Biochemical and Biophysical Research Communications·F J WiebelM Lambiotte

Citations

Apr 1, 1994·Cell Biology and Toxicology·P KremersJ P Thome
Nov 1, 1993·The International Journal of Biochemistry·J T BorlakogluC R Wolf
Jan 1, 1987·Pharmacology & Therapeutics·W Klinger
Dec 1, 1988·Molecular and Cellular Endocrinology·J M MathisR A Prough
Nov 1, 1996·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·J H ParmentierP Kremers
Jun 1, 1988·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·C Guguen-GuillouzoA Guillouzo

Related Concepts

Aldrin epoxidase
Testosterone 7-alpha-hydroxylase, hamster
Xenobiotic Monooxygenases
Cytochrome P-450 Oxygenase
Oradexon
Fetal Structures
Mixed Function Oxygenases
Liver
August Rats
Steroid Hydroxylases

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