The expression of hyperpolarization activated cyclic nucleotide gated (HCN) channels in the rat ovary are dependent on the type of cell and the reproductive age of the animal: a laboratory investigation.

Reproductive Biology and Endocrinology : RB&E
John YehArmando Arroyo

Abstract

Aim of this study was to test the hypothesis that levels of hyperpolarization activated cyclic nucleotide gated channels 1 to 4 (HCN1-4) are linked to the reproductive age of the ovary. Young, adult, and reproductively aged ovaries were collected from Sprague-Dawley rats. RT-PCR and western blot analysis of ovaries was performed to investigate the presence of mRNA and total protein for HCN1-4. Immunohistochemistry with semiquantitative H score analysis was performed using whole ovarian histologic sections. RT-PCR analysis showed the presence of mRNA for HCN1-4. Western blot analysis revealed HCN1-3 proteins in all ages of ovarian tissues. Immunohistochemistry with H score analysis demonstrated distinct age-related changes in patterns of HCN1-3 in the oocytes, granulosa cells, theca cells, and corpora lutea. HCN4 was present only in the oocytes, with declining levels during the reproduction lifespan. The evidence presented here demonstrates cell-type and developmental age patterns of HCN1-4 channel expression in rat ovaries. Based on this, we hypothesize that HCN channels have functional significance in rat ovaries and may have changing roles in reproductive aging.

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Citations

Jul 29, 2011·Kidney International·Rolando Carrisoza-GaytánLaura I Escobar
Aug 30, 2016·Biochemical and Biophysical Research Communications·Rafael ZúñigaLeandro Zúñiga
Aug 21, 2018·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Iman M Al-NaggarPhillip P Smith
Aug 18, 2021·Progress in Biophysics and Molecular Biology·Patrizia BenzoniAnnalisa Bucchi

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

SPSS

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