The expression of I-A correlates with the uptake of interferon-gamma by macrophages

European Journal of Immunology
A Celada, R A Maki

Abstract

The current studies were designed to examine some of the requirements for I-A expression when macrophages (M phi) were treated with interferon-gamma (IFN-gamma). In order to define the minimum time required for IFN-gamma to induce surface expression of I-A antigen on bone marrow-derived M phi, cells were incubated with IFN-gamma for varying lengths of time, washed and thereafter incubated for 72 h before assaying I-A surface expression. Using saturating amounts of IFN-gamma (300 IRU/ml), we found that between 0 and 30 min of IFN-gamma treatment there is a direct correlation between the length of treatment and the level of I-A surface expression. When the steady state level of RNA for the I-A beta gene was assayed, a low level of I-A beta RNA was seen in cells treated for 10 min with saturating amounts of IFN-gamma (300 IRU/ml) while a 30-min or 60-min exposure of cells to the same concentration of IFN-gamma resulted in a steady increase in the level of I-A beta RNA. Similar results were found when we measured the levels of RNA for the tumor necrosis factor and C3 complement genes, both of which are induced by IFN-gamma in M phi. M phi treated with low amounts of IFN-gamma (3 IRU/ml) for 30 min do not express cell surface I-A. C...Continue Reading

References

Dec 1, 1985·The Journal of Clinical Investigation·A CeladaR D Schreiber
Jun 1, 1987·Immunological Reviews·M RubinsteinD G Fischer
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J SancéauC Vaquero
Jul 1, 1984·The Journal of Experimental Medicine·A CeladaR D Schreiber

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Citations

Dec 30, 1999·Microbes and Infection·J LloberasA Celada
Oct 13, 1995·The Journal of Biological Chemistry·F E BorràsA Celada
Apr 22, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Carlos SebastiánAntonio Celada
Oct 31, 2018·Nature Nanotechnology·Ji WangXi Xie
Nov 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·J XausA Celada
Nov 1, 1993·Molecular and Cellular Biology·I S ThoreyR G Oshima
Apr 1, 1993·Molecular and Cellular Biology·N NeznanovR G Oshima

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