The expression of neurokinin-1 and preprotachykinin-1 in breast cancer cells depends on the relative degree of invasive and metastatic potential.

Clinical & Experimental Metastasis
Tammy A CastroPranela Rameshwar

Abstract

Breast cancer has a predilection for metastasis to the bone marrow. The preprotachykinin-I (PPT-I) gene has a central role in the early migration of breast cancer cells into the bone marrow, making this organ a latent repository of the cancer cells. This study investigated whether the invasive and metastatic potential of breast cancer cells correlate with the expression of the PPT-I gene and the receptors for its peptides, neurokinin-1 (NK-1) and NK-2. The studies compared cells that are non-tumorigenic (MCF12A), low metastatic and invasive potential (MCF7), and sublines of MCF with increased invasive and metastatic potential (LCC1 and LCC2). LCC2, but not LCC1 is tamoxifen resistant. Quantitative RT-PCR showed increased expression of PPT-I, NK-1 and NK-2 mRNA LCC1 and LCC2. MCF7 required stimulation by phorbol ester for NK-1 induction. The levels of NK-2 mRNA were significantly increased in LCC2. Clonogenic assays with specific receptor antagonists showed a predominant role for NK-2 in the proliferation of both LCC1 and LCC2. While the growth rate of LCC1 and LCC2 were similar, the latter showed increased migration. Use of a nude mouse model confirmed higher metastatic potential of LCC2, including increased migration to region...Continue Reading

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Citations

May 1, 2012·TheScientificWorldJournal·Marisa RossoMichael Berger
May 16, 2013·Cancer Metastasis Reviews·Ming ShiNing Guo
May 24, 2011·Journal of Cellular Physiology·Cristina MayordomoVanessa Almendro
Oct 1, 2015·BioMed Research International·Susana Garcia-Recio, Pedro Gascón
May 28, 2011·Biochimica Et Biophysica Acta·Mario MancinoVanessa Almendro
Apr 12, 2020·Molecular Biology Reports·Shima LorestaniSeyed Isaac Hashemy
Apr 20, 2013·Oncology Letters·Sha LiDongwei Gao
Jun 7, 2013·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Derick Okwan-DuoduRoberto Diaz
Jan 2, 2019·Cancer Cell International·Silvia Gutierrez, M Danilo Boada

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