PMID: 8938590Oct 1, 1996Paper

The expression of parathyroid hormone and parathyroid hormone-related protein in developing rat parathyroid glands

Journal of Molecular Endocrinology
J TucciF Beck

Abstract

Secretion of parathyroid hormone-related protein (PTHrP) by sheep fetal parathyroid glands is reported to be an important factor in the maintenance of a placental calcium pump. The aim of the present study was to determine whether the developing rat parathyroid glands express PTHrP or parathyroid hormone (PTH), or both. Hybridisation histochemistry was used to detect transcription of PTHrP and PTH in serial paraffin sections through the 12.5- and 13.5-day rat embryo parathyroid anlage, as well as in sections through the 17.5-day embryonic and adult parathyroid glands. Results show strong expression of PTH in the 13.5-day embryonic parathyroid anlage, as well as in the parathyroid gland of the 17.5-day embryo and adult. Transcription of the PTHrP gene was not detected. The more sensitive technique of reverse transcription PCR was then performed. The pharyngeal region of 11.5-, 12.5- and 13.5-day rat embryos was dissected out and, at each stage, RNA was extracted from these tissues, as well as pooled tissues from the rest of the embryo. RNA that had been extracted from adult thyroid/parathyroid tissue was also tested. After reverse transcription, the resulting cDNAs were amplified by PCR (50 cycles) using specific PTH and PTHrP p...Continue Reading

Citations

Nov 11, 1998·Clinical and Experimental Pharmacology & Physiology. Supplement·F Beck
Apr 18, 2001·The Journal of Clinical Investigation·C S KovacsH M Kronenberg
Jun 24, 1998·The Journal of Clinical Investigation·C S KovacsH M Kronenberg
Mar 15, 2014·Endocrine·Sergio FabbriMaria Luisa Brandi
Jan 25, 2014·Scandinavian Journal of Clinical and Laboratory Investigation·Anders NordholmEwa Lewin
Mar 26, 2015·Frontiers in Endocrinology·Jason S W OnLeo T O Lee
May 24, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Esmond J Sanders, Steve Harvey

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