PMID: 22568080May 10, 2012Paper

The expression of recombinant factor VII variants is influenced by different cells for transfection using expression studies

Rinsho byori. The Japanese journal of clinical pathology
Megumi Yoshihiro, Osamu Takamiya

Abstract

To investigate the influence of different transfected cells using expression studies of dysfunctional factor VII variants, we expressed recombinant FVII using COS-1 cells, CHO-K1 cells, BHK cells and HEK293 cells. The mutant type F7 DNAs with Leu-48Pro in the prepeptide domain, with Ser126Phe in the EGF-2 domain and with Gly354Cys in the catalytic domain were transfected in the four kinds of cell lines. Additionally, FVII Ser126Tyr and Ser126Gly were expressed to investigate different substitutions from Phe in position 126 in FVII. The FVII:Ag of recombinant FVII Leu-48Pro in conditioned medium, which were expressed in COS-1 cells, CHO-K1 cells, BHK cells and HEK293 cells, were 37.9% 68.0%, 11.7% and 9.2% respectively, when wild-type FVII was defined as 100%. The levels of FVII:Ag in cell lysates were 26.2%, 44.9%, 25.6% and 50.5%, respectively. The FVII:C of recombinant FVII Gly354Cys in conditioned medium were 3.7%, 3.5%, 1.5% and 1.7%, respectively. The levels of FVII:Ag were similar to FVII:C. The FVII:C of both recombinant FVII Ser126Phe and Ser126Tyr in conditioned medium of all four cell lines were considerably lower than that of FVII wild type. However, levels of FVII:Ag in conditioned medium of recombinant FVII S126G w...Continue Reading

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved