The expression of the genes for fructosamine-3-kinase and fructosamine-3-kinase-related protein appears to be constitutive and unaffected by environmental signals

Biochemical and Biophysical Research Communications
James R ConnerB S Szwergold

Abstract

Fructosamine-3-kinase (FN3K) and the more recently discovered fructosamine-3-kinase related protein (FN3KRP) appear to protect proteins from nonenzymatic glycation. To elucidate the patterns of transcriptional regulation of these two genes, we performed in silico comparisons of their promoters along with real-time PCR assays of their expression in a variety of human tissues. Both promoters were TATA-less and CAAT-less, and contained several homologous CpG islands and Sp1 binding sites. The genes were expressed in all human tissues examined, with FN3K showing significantly higher levels in organs susceptible to nonenzymatic glycation and diabetic complications. Cultured fibroblasts treated with conditions mimicking the hormonal and biochemical profile of the diabetic state showed no changes in FN3K and FN3KRP expression relative to untreated cells. These data suggest that FN3K and FN3KRP act as protein repair enzymes and are expressed constitutively in human cells independently of some of the variables altered in the diabetic state.

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Citations

Mar 30, 2007·Diabetes, Obesity & Metabolism·N Ahmed, P J Thornalley
Feb 4, 2011·Clinical Chemistry and Laboratory Medicine : CCLM·Lorena MoscaAndrea Mosca
Oct 23, 2010·Amino Acids·Emile Van SchaftingenMaria Veiga-da-Cunha
Aug 10, 2007·Biochemical and Biophysical Research Communications·Benjamin SzwergoldKerry Loomes
Jul 23, 2005·Annals of the New York Academy of Sciences·Benjamin S SzwergoldPaul J Beisswenger
Mar 14, 2017·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Shin-Ichi InabaTakashi Izumi
Mar 13, 2008·Oncology·Maria Gabriella CarusoGiovanni Misciagna
Sep 10, 2015·Clinical Chemistry and Laboratory Medicine : CCLM·Francesca AvemariaAndrea Mosca
May 8, 2013·Clinical Chemistry and Laboratory Medicine : CCLM·Anne HellwigThomas Henle
Mar 14, 2019·Pain Medicine : the Official Journal of the American Academy of Pain Medicine·Larissa J StrathRobert E Sorge
Sep 10, 2020·Journal of Clinical Medicine·Sander De BruyneJoris R Delanghe
Oct 31, 2017·Biogerontology·Frank HauseAlexander Navarrete Santos
Oct 22, 2020·Pain and Therapy·Ashish S KaushikRobert E Sorge
May 1, 2021·International Journal of Molecular Sciences·Sander De BruyneJoris R Delanghe

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