PMID: 11934567Apr 6, 2002Paper

The expression of the Helicobacter pylori genes ureA and nap is higher in vivo than in vitro as measured by quantitative competitive reverse transcriptase-PCR

FEMS Immunology and Medical Microbiology
Kristina BlomIngrid Bölin

Abstract

The expression of the virulence-associated genes ureA, encoding the urease subunit A, and nap, encoding the neutrophil activating protein, in Helicobacter pylori grown both in the stomach of C57/Bl6 mice and in Brucella broth was quantified by quantitative competitive reverse transcriptase-PCR using a homologous RNA standard (competitor) and an external standard (16S rRNA). The results showed that the ureA and nap transcripts were increased up to 15 and 80 times, respectively, in vivo compared to in vitro. The transcription of ureA and nap also differed in that ureA showed highest expression early in infection in mice whereas nap transcription was variable throughout the 18-week infection period.

References

Jun 1, 1995·Infection and Immunity·D J EvansP R Kvietys
Sep 1, 1994·The American Journal of Medicine·R J Hopkins, J G Morris
Dec 1, 1993·Epidemiology and Infection·G BodeP Malfertheiner
Jul 25, 1997·The Journal of Biological Chemistry·S TenebergK A Karlsson
Feb 20, 1998·Clinica Chimica Acta; International Journal of Clinical Chemistry·J CaoS Mårdh
May 30, 1998·Clinical and Diagnostic Laboratory Immunology·A MattssonA M Svennerholm
Jan 12, 1999·Journal of Bacteriology·G L ScoarughiP Donini
Jan 23, 1999·BioTechniques·W M FreemanK E Vrana
May 21, 1999·Science·A CovacciR Rappuoli
Sep 8, 1999·Microbiology and Molecular Biology Reviews : MMBR·P DoigT J Trust

❮ Previous
Next ❯

Citations

Apr 13, 2005·Journal of Microbiological Methods·Yvonne RousselSoad Tabaqchali
Mar 26, 2003·Infection and Immunity·Kimmo VirtanevaJames M Musser
Jul 30, 2005·FEMS Immunology and Medical Microbiology·Philip James BerginQuiding-Järbrink Marianne

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.