The expression of TNF alpha by human muscle. Relationship to insulin resistance

The Journal of Clinical Investigation
M SaghizadehP A Kern

Abstract

TNFalpha is orverexpressed in the adipose tissue of obese rodents and humans, and is associated with insulin resistance. To more closely link TNF expression with whole body insulin action, we examined the expression of TNF by muscle, which is responsible for the majority of glucose uptake in vivo. Using RT-PCR, TNF was detected in human heart, in skeletal muscle from humans and rats, and in cultured human myocytes. Using competitive RT-PCR, TNF was quantitated in the muscle biopsy specimens from 15 subjects whose insulin sensitivity had been characterized using the glucose clamp. technique. TNF expression in the insulin resistant subjects and the diabetic patients was fourfold higher than in the insulin sensitive subjects, and there was a significant inverse linear relationship between maximal glucose disposal rate and muscle TNF (r = -0.60, P < 0.02). In nine subjects, muscle cells from vastus lateralis muscle biopsies were placed into tissue culture for 4 wk, and induced to differentiate into myotubes. TNF was secreted into the medium from these cells, and cells from diabetic patients expressed threefold more TNF than cells from nondiabetic subjects. Thus, TNF is expressed in human muscle, and is expressed at a higher level i...Continue Reading

References

Jul 1, 1976·Clinics in Endocrinology and Metabolism·E A Sims
Jul 30, 1992·The New England Journal of Medicine·C Grunfeld, K R Feingold
May 24, 1990·The New England Journal of Medicine·C BouchardG Fournier
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J S PattonC Grunfeld
Jun 15, 1988·Journal of the National Cancer Institute·S H SocherA Oliff
Oct 1, 1994·The Journal of Clinical Investigation·G S HotamisligilB M Spiegelman
May 1, 1995·The Journal of Clinical Investigation·G S HotamisligilB M Spiegelman
Nov 1, 1994·Diabetes·G S Hotamisligil, B M Spiegelman
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·G S HotamisligilB M Spiegelman

❮ Previous
Next ❯

Citations

Sep 1, 2012·Lasers in Medical Science·Vítor S HentschkePedro Dal Lago
May 12, 2011·Molecular Biology Reports·M HedayatiF Azizi
Jun 3, 2005·Current Diabetes Reports·Rebecca Parish, Kitt Falk Petersen
Oct 24, 2007·Biological Trace Element Research·Satish Kumar Taneja, Reshu Mandal
Jan 16, 2014·Indian Journal of Clinical Biochemistry : IJCB·Jai PrakashNeena Srivastava
Jun 21, 2005·The International Journal of Biochemistry & Cell Biology·P Christian Schulze, Ulrike Späte
Oct 3, 2006·NeuroRx : the Journal of the American Society for Experimental NeuroTherapeutics·Frederick M IveyRichard F Macko
Jan 17, 2004·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Weidong YinKechao Tang
Jun 17, 2000·Atherosclerosis·E RaynaudJ Mercier
Jul 22, 1998·Journal of Immunological Methods·B M DarkeJ D Fox
Aug 7, 2002·International Journal of Cardiology·Joseph Walsmith, Ronenn Roubenoff
Mar 14, 2002·Diabetes Research and Clinical Practice·Gábor WinklerIstván Karádi
Jun 16, 1999·Diabetes Research and Clinical Practice·M FukuzawaT Toyota
Jun 18, 2002·Journal of Ethnopharmacology·M C Sabu, Ramadasan Kuttan
Sep 6, 2003·Journal of the American College of Cardiology·Stephan GielenRainer Hambrecht
Oct 13, 1998·Journal of the American College of Cardiology·U N RiedeH Drexler
Nov 13, 2004·The Journal of Nutritional Biochemistry·J M ArgilésF J López-Soriano
Jul 6, 2000·Trends in Endocrinology and Metabolism : TEM·D E Moller
Sep 16, 2000·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·L Groop
Mar 8, 2012·Nature Medicine·Olivia Osborn, Jerrold M Olefsky
Jul 8, 1999·Journal of Internal Medicine·G S Hotamisligil
Aug 8, 2001·Journal of Internal Medicine·L Groop, M Orho-Melander
Nov 13, 2001·Diabetic Medicine : a Journal of the British Diabetic Association·M T CoughlanG E Rice
Feb 2, 2000·Proceedings of the Society for Experimental Biology and Medicine·C Qi, P H Pekala
Oct 18, 2012·Seminars in Reproductive Medicine·Antonio E Frias, Kevin L Grove
Jul 22, 1999·The New England Journal of Medicine·P R Shepherd, B B Kahn

❮ Previous
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