The expression of Wnt4 is regulated by estrogen via an estrogen receptor alpha-dependent pathway in rat pituitary growth hormone-producing cells.

Acta Histochemica Et Cytochemica
Takashi MiyakoshiRobert Yoshiyuki Osamura

Abstract

Wnt signaling is important in many aspects of cell biology and development. In the mouse female reproductive tract, Wnt4, Wnt5a, and Wnt7a show differential expression during the estrus cycle, suggesting that they participate in female reproductive physiology. Although the pituitary is a major gland regulating reproduction, the molecular mechanism of Wnt signaling here is unclear. We elucidated the subcellular distribution of Wnt4 in the pituitary of estrogen-treated ovariectomized female rats. Expression of Wnt4 mRNA increased dramatically, particularly in proestrus compared with estrus and metestrus. Wnt4 protein was observed in the cytoplasm of almost all growth hormone (GH)-producing cells and in only a few thyroid-stimulating hormone beta (TSHbeta)-producing cells. In rat GH-producing pituitary tumor (MtT/S) cells, estrogen-induced expression of Wnt4 mRNA was completely inhibited by estrogen receptor antagonist ICI 182,780 in vitro. Thus, rat pituitary GH cells synthesize Wnt4 and this is induced by estrogen mediated via an estrogen receptor alpha-dependent pathway.

References

Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·G G KuiperJ A Gustafsson
Jun 1, 1997·Molecular Endocrinology·K M ScullyM G Rosenfeld
Aug 8, 1997·The Journal of Biological Chemistry·S M CowleyM G Parker
Feb 7, 1998·Genes & Development·K M Cadigan, R Nusse
Jun 17, 1998·Genes & Development·M TreierM G Rosenfeld
Jun 20, 2000·Trends in Genetics : TIG·M KühlR T Moon
Oct 18, 2003·Analytical and Bioanalytical Chemistry·Stefan O Mueller
Jan 30, 2004·The Journal of Biological Chemistry·Tamar GolanArnona Gazit
Mar 3, 2004·Molecular Interventions·Jason Matthews, Jan-Ake Gustafsson
Apr 10, 2004·Development·Mathias MericskayDavid Sassoon
Sep 11, 2004·Molecular Endocrinology·Xiaonan HouSanjoy K Das
Dec 8, 2004·Developmental Biology·Katherine Jeays-WardAmanda Swain
Dec 25, 2004·Endocrinology·Gwen V ChildsBrandy Whitehead-Johnson
Mar 23, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·Seiichi KatayamaKenji Nagai
Oct 4, 2006·BMC Developmental Biology·Hongshi YuMarilyn B Renfree
Dec 5, 2006·Developmental Cell·Ritsuko TakadaShinji Takada
Dec 26, 2006·Cellular Signalling·Akira KikuchiShosei Kishida
Jul 20, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Masuko Katoh, Masaru Katoh
Dec 15, 2007·Endocrinology·Marianne S ElstonKerrie L McDonald
Mar 21, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Mary Anne PotokSally A Camper
Jul 19, 2008·Acta Histochemica Et Cytochemica·Noboru EgashiraRobert Y Osamura
Nov 27, 2008·Endocrine Pathology·Takashi MiyakoshiRobert Yoshiyuki Osamura
Jan 23, 2009·American Journal of Physiology. Endocrinology and Metabolism·S ZárateA Seilicovich

❮ Previous
Next ❯

Citations

Dec 15, 2015·American Journal of Reproductive Immunology : AJRI·Renjini A Padmanabhan, Malini Laloraya
Mar 26, 2013·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Jiwei BaiYazhuo Zhang
Jan 10, 2012·International Journal of Cancer. Journal International Du Cancer·Dominique Nadine MarkowskiJörn Bullerdiek
Dec 19, 2013·Behavioral and Brain Functions : BBF·Jia LuoFeng Jin
Mar 25, 2014·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Sara Ferrando-MartínezManuel Leal
Oct 19, 2014·Molecular Genetics and Genomics : MGG·Sunil K HalderAyman Al-Hendy
Feb 28, 2018·Journal of Medicinal Food·Jeremy E Davis, Darcie Hastings
May 22, 2013·Endocrine-related Cancer·T J G ChambersJ R E Davis
Nov 29, 2013·Molecular Endocrinology·Dimiter AvtanskiSally Radovick
Sep 17, 2020·BMC Molecular and Cell Biology·Melanie K StewartAndrew J Pask
Aug 24, 2018·International Journal of Molecular Sciences·Sofia MakievaPaola Viganò
Nov 12, 2020·International Journal of Molecular Sciences·Melanie K StewartAndrew J Pask
Jan 20, 2011·Journal of Cell Science·Adam GilesJulian R E Davis
May 18, 2021·Molecular and Cellular Biochemistry·Malak El SabehMostafa A Borahay

❮ Previous
Next ❯

Methods Mentioned

BETA
glycosylation
PCR
electrophoresis

Software Mentioned

Match
Primer3
StatMate
Methyl Primer Express

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.