The expression pattern of PFKFB3 enzyme distinguishes between induced-pluripotent stem cells and cancer stem cells

Oncotarget
Artur Cieślar-PobudaEmilia Wiechec

Abstract

Induced pluripotent stem cells (iPS) have become crucial in medicine and biology. Several studies indicate their phenotypic similarities with cancer stem cells (CSCs) and a propensity to form tumors. Thus it is desirable to identify a trait which differentiates iPS populations and CSCs. Searching for such a feature, in this work we compare the restriction (R) point-governed regulation of cell cycle progression in different cell types (iPS, cancer, CSC and normal cells) based on the expression profile of 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase3 (PFKFB3) and phosphofructokinase (PFK1). Our study reveals that PFKFB3 and PFK1 expression allows discrimination between iPS and CSCs. Moreover, cancer and iPS cells, when cultured under hypoxic conditions, alter their expression level of PFKFB3 and PFK1 to resemble those in CSCs. We also observed cell type-related differences in response to inhibition of PFKFB3. This possibility to distinguish CSC from iPS cells or non-stem cancer cells by PFKB3 and PFK1 expression improves the outlook for clinical application of stem cell-based therapies and for more precise detection of CSCs.

References

Nov 3, 1989·Science·A B Pardee
Jan 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J CampisiA B Pardee
Nov 2, 2001·Nature·T ReyaI L Weissman
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Apr 12, 2002·The Journal of Biological Chemistry·Huasheng LuAjay Verma
Mar 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·Muhammad Al-HajjMichael F Clarke
Feb 24, 1956·Science·O WARBURG
Aug 24, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Hidenori BandoTakao Koike
Feb 21, 2006·Cancer Research·Max S WichaGabriela Dontu
May 31, 2008·Archivum Immunologiae Et Therapiae Experimentalis·Sabine Hombach-KlonischMarek Los
Sep 9, 2008·Trends in Molecular Medicine·Thomas KlonischMarek Los
Mar 10, 2009·Biochemical and Biophysical Research Communications·Matthieu SchoenhalsBernard Klein
May 21, 2009·Experimental and Molecular Pathology·Abdullah YalcinJason Chesney
Jul 11, 2009·Nature Biotechnology·Kyoko MiuraShinya Yamanaka
Sep 1, 2009·Cell Stem Cell·Yoshinori YoshidaShinya Yamanaka
Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Angeles AlmeidaSalvador Moncada
Dec 21, 2010·Cancer Research·Igor ShatsJoseph R Nevins
Mar 16, 2011·Proceedings of the National Academy of Sciences of the United States of America·Slavica TudzarovaSalvador Moncada
Aug 4, 2011·Clinics·Suely Kazue Nagahashi Marie, Sueli Mieko Oba Shinjo
Nov 19, 2011·Cold Spring Harbor Symposia on Quantitative Biology·I MorK H Vousden
Nov 23, 2011·Proceedings of the National Academy of Sciences of the United States of America·Sergio L ColomboSalvador Moncada
Nov 26, 2011·The Biochemical Journal·Laura NovellasdemuntRamon Bartrons
May 18, 2012·Journal of Translational Medicine·Sucheta TelangJason Chesney
Oct 2, 2012·Differentiation; Research in Biological Diversity·Jonathan RakarGunnar Kratz
May 30, 2013·Development·Kazutoshi Takahashi, Shinya Yamanaka

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Citations

Apr 8, 2017·Archivum Immunologiae Et Therapiae Experimentalis·Wiem ChaabaneMarek J Łos
Feb 13, 2016·Journal of Cellular and Molecular Medicine·Weidong BingXiaolu Bi
Jul 16, 2016·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Amancio Carnero, Matilde Lleonart
Mar 17, 2016·The International Journal of Biological Markers·Chen-Min SunXu-Dong Yao
May 5, 2017·International Journal of Molecular Sciences·Emilia WiechecKarin Roberg
Dec 1, 2017·International Journal of Molecular Sciences·Aleksanra SkubisMarek J Łos
Jul 10, 2018·Expert Opinion on Therapeutic Targets·Ramon BartronsÀurea Navarro-Sabaté
Dec 31, 2019·Stem Cells International·Jilan LiuFeng Jin
Mar 23, 2017·Macromolecular Bioscience·Peter C SherrellMehrdad Rafat
Dec 22, 2017·Signal Transduction and Targeted Therapy·Linlin ShiWeidong Han
Sep 4, 2016·Journal of Translational Medicine·Giulia M StellaSilvia Benvenuti
Jan 8, 2017·Journal of Experimental & Clinical Cancer Research : CR·Hui-Min LiJun Jia

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Methods Mentioned

BETA
flow cytometry
Transfection
Assay
fluorescence-activated cell sorting
FACS
PCR

Software Mentioned

Kaluza
Image J

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