The expressions of NLRP1, NLRP3, and AIM2 inflammasome complexes in the contusive spinal cord injury rat model and their responses to hormonal therapy.

Cell and Tissue Research
Jamal MajidpoorMohammad Taghi Joghataei

Abstract

Spinal cord injury (SCI) is a devastating condition with a growing incidence in developing countries. The activity of inflammasome complexes initiates neuroinflammation, which is a key player in SCI pathogenesis. Here, NLRP1, NLRP3, and absent in melanoma 2 (AIM2) inflammasome complexes were assessed in the contusive (T6) SCI rats for their expression profiles and their response to hormonal therapy (10 mg/kg melatonin or 25 μg/kg 17β-estradiol [E2] every 12 h until 72 h). Two phases was considered in this study: the dominant time of inflammasome activation, which was 72 h post-SCI and the response from each complex to hormonal therapy at this time. Gene and protein expressions of NLRP1, NLRP3, AIM2, ASC, and caspase-1 were evaluated by real-time PCR (for gene analysis), western blot, and immunohistochemistry (IHC), and biochemical presence of IL-18 and IL-1β in spinal cord tissue homogenates was analyzed by enzyme-linked immunosorbent assay (ELISA). The whole inflammasome complexes showed high expressions in the SCI group, while after hormonal therapy, these alterations were counteracted, which were more conspicuous for the NLRP1 and NLRP3. Melatonin had no predilection over E2 for such effect. Finally, the expression profile o...Continue Reading

References

Dec 25, 2002·Trends in Pharmacological Sciences·Phillip G Popovich, T Bucky Jones
Jun 16, 2006·Journal of Neurotrauma·Pimonporn ChaovipochCandace L Floyd
Mar 28, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Juan Pablo de Rivero VaccariRobert W Keane
Jan 9, 2014·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Stephanie E AdamczakRobert W Keane
Jan 9, 2014·Nature Reviews. Neuroscience·John G WalshChristopher Power
Mar 4, 2014·International Immunopharmacology·Yun-peng LuoMing-yan Zhao
Mar 10, 2015·The Journal of Steroid Biochemistry and Molecular Biology·Alexander Slowik, Cordian Beyer
May 31, 2015·Translational Research : the Journal of Laboratory and Clinical Medicine·Juan Pablo de Rivero VaccariRobert W Keane
Jul 15, 2016·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Monika FleshnerSteven F Maier
Jan 28, 2017·Molecular Neurobiology·Adib ZendedelCordian Beyer
Apr 22, 2017·Journal of Cellular and Molecular Medicine·Zheng LiJianxiong Shen
Oct 27, 2017·Journal of Neuroinflammation·Wu JiangLiulong Zhu
Nov 7, 2017·International Journal of Endocrinology·Gaia FaveroRita Rezzani
Nov 19, 2017·Journal of Cellular Physiology·Keywan MortezaeeAdib Zendedel
Sep 27, 2018·Journal of Cellular Biochemistry·Guanghui XuBaoqing Yu

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Citations

Jul 3, 2021·Antioxidants·Burak Ibrahim AriozSermin Genc
Aug 18, 2021·International Journal of Immunopathology and Pharmacology·Yongxiang YuXiaohui Dong

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

SPSS

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