The extracellular domains of E- and N-cadherin determine the scattered punctate localization in epithelial cells and the cytoplasmic domains modulate the localization

Journal of Biochemistry
Chisa OzakiShintaro T Suzuki

Abstract

The accumulation of classical cadherins is essential for their function, but the mechanism is poorly understood. Hence, we investigated the accumulation of E- and N-cadherin and the formation of cell junctions in epithelial cells. Immunostaining revealed a scattered dot-like accumulation of E- and N-cadherin throughout the lateral membrane in MDCK II and other epithelial cells. Mutant E-cadherin lacking the beta-catenin binding site accumulated granularly at cell-cell contact sites and showed weak cell aggregation activity in cadherin-deficient epithelial cells, MIA PaCa2 cells. Mutant E-cadherin lacking the p120-catenin binding site exhibited scattered punctate accumulation and strong cell adhesion activity in MIA PaCa2 cells. Electron microscopy demonstrated that MIA PaCa2 transfectants of E-cadherin containing beta-catenin binding site formed adherens junction, whereas E-cadherin lacking the binding site did not. Mutant N-cadherins showed accumulation properties similar to those of corresponding mutant E-cadherins. Moreover, wild type and mutant N-cadherin lacking the p120-catenin binding site showed subapical accumulation in polarized DLD-1 cells, whereas mutant N-cadherin lacking beta-catenin binding site did not. These re...Continue Reading

References

Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M OzawaR Kemler
Mar 23, 1995·Nature·L ShapiroW A Hendrickson
May 1, 1993·Cell Adhesion and Communication·G M Edelman
Feb 1, 1993·Current Opinion in Cell Biology·D R Garrod
Oct 1, 1995·Current Opinion in Cell Biology·M Takeichi
Jan 24, 1998·The Journal of Biological Chemistry·R B HazanD L Rimm
Jan 12, 2000·The Journal of Cell Biology·M A ThoresonA B Reynolds
Sep 7, 2001·Current Opinion in Cell Biology·A Nagafuchi
May 1, 1963·The Journal of Cell Biology·M G FARQUHAR, G E PALADE
May 21, 2004·American Journal of Physiology. Cell Physiology·Eveline E Schneeberger, Robert D Lynch
Sep 7, 2004·The Journal of Biological Chemistry·Yoji MurataTakeshi Yagi
Dec 3, 2004·Molecular Biology of the Cell·Wei YuMirjam M P Zegers
Dec 6, 2005·Cell·Soichiro YamadaW James Nelson
May 26, 2006·Investigative Ophthalmology & Visual Science·Yong-Ha YounJanice M Burke
Dec 13, 2006·Nature Cell Biology·Yoshiko Kametani, Masatoshi Takeichi
Dec 7, 2007·Journal of Cell Science·Yayoi Miyashita, Masayuki Ozawa

❮ Previous
Next ❯

Citations

Nov 22, 2014·Journal of Biochemistry·Yusuke IzutaShintaro T Suzuki
Aug 15, 2017·Experimental Cell Research·Deepika VermaSusan Z Hua
May 5, 2012·Trends in Cell Biology·Julia BraschLawrence Shapiro

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Soonjin HongSergey M Troyanovsky
The Journal of Cell Biology
M G FARQUHAR, G E PALADE
Cold Spring Harbor Perspectives in Biology
Lawrence Shapiro, William I Weis
Cold Spring Harbor Perspectives in Biology
Takashi WatanabeKozo Kaibuchi
© 2022 Meta ULC. All rights reserved