The extracellular part of zeta is buried in the T cell antigen receptor complex

Immunology Letters
Susana MinguetWolfgang W A Schamel

Abstract

The zeta chain is a key component of the T cell antigen receptor (TCR-CD3) complex, required for the expression of the receptor on the cell surface. It contains an extremely small extracellular (EC) part of nine amino acids. Interestingly, the length, but not the sequence, of the zeta EC has been highly conserved through evolution. Here, we examined the effect of increasing the length of human zeta EC on TCR-CD3 assembly and surface expression. Appending a 30 kDa polypeptide to the N-terminus of zeta completely abolished assembly and transport of the TCR-CD3 to the cell surface. Addition of only 17 amino acids, including the HA-tag (HAzeta), strongly reduced the efficiency of TCR-CD3 assembly and led to reduced expression on the surface, suggesting that the short zeta EC region is located within the receptor complex. In Blue Native gels (BN-PAGE) these receptors had a normal size, indicating that they have a stoichiometry of alphabetagammaepsilondeltaepsilonzetazeta. In resting TCR-CD3s the HA-tag, and thus the zeta EC region, was not accessible for anti-HA antibody binding, demonstrating that it was indeed buried in a cavity within the receptor complex. However, prolonged stimulation with antigen permitted the access of the an...Continue Reading

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Citations

Mar 20, 2010·Cell Communication and Signaling : CCS·Mahima SwamyWolfgang Wa Schamel
Jan 22, 2008·Immunology Letters·Susana MinguetWolfgang W A Schamel
Dec 25, 2009·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·John S BridgemanDavid E Gilham
May 9, 2019·Nature Communications·Patrick A BaeuerleRobert Hofmeister
May 21, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·John S BridgemanDavid E Gilham
Aug 14, 2019·Immunological Reviews·Wolfgang W SchamelSusana Minguet

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