PMID: 7011317Sep 1, 1980Paper

The extraction of a neutral metalloproteinase from the involuting rat uterus, and its action on cartilage proteoglycan

The Biochemical Journal
A Sellers, J F Woessner

Abstract

1. Homogenates of rat uteri removed 1 and 2 days post partum were centrifuged at 6000 g. Both pellets and supernatants degraded Azocoll, a general proteinase substrate, at pH 7.5. More than 80% of the total activity was in the pellet fraction. 2. Part of the pellet activity was in a latent form. Trypsin and 4-aminophenylmercuric acetate (a thiol-blocking agent) both activated this latent form, indicating that it is an enzyme--inhibitor complex. An endogenous serine proteinase activated part of the latent enzyme during the assay. 3. The enzyme activity was low before parturition and after involution; it was highest during the first 2 days post partum, when the largest losses of uterine wet weight and matrix macromolecules occur. 4. Up to 70% of the enzyme in the pellets was extracted by heating at 60 degrees C for 4 min in 0.1 M-CaCl2/0.05 M-Tris/HCl, pH 7.5. Approx. 30% of the extracted enzyme was still latent. 5. The extracted enzyme was a metalloproteinase, since it was inhibited completely by 1,10-phenanthroline, but not by inhibitors of thiol or serine proteinases. 6. The enzyme was further purified 15--30-fold by gel chromatography and precipitation with (NH4)2SO4. The apparent molecular weight, estimated by gel filtration...Continue Reading

Citations

Jul 23, 1999·Annals of the New York Academy of Sciences·J F Woessner
Jun 26, 2012·Research in Veterinary Science·Hiromichi TamadaTsutomu Sawada
Oct 11, 1990·Matrix : Collagen and Related Research·D D DeanD S Howell
Jun 28, 2000·American Journal of Obstetrics and Gynecology·E MaymonD E Seubert
May 18, 2016·Cellular and Molecular Life Sciences : CMLS·Aleta Pupovac, Ronald Sluyter
Mar 26, 2004·American Journal of Obstetrics and Gynecology·Fabián Arechavaleta-VelascoSamuel Parry
Apr 1, 1991·Journal of Cellular Biochemistry·A OttleczB Starcher
Jan 24, 2007·Physiological Reviews·Kendra J GreenleeFarrah Kheradmand
Jan 1, 1996·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·J F Woessner
Jun 7, 2019·Journal of Zhejiang University. Science. B·Zi-Huan ZhengXiao-Dong Zheng
Aug 1, 1982·Arthritis and Rheumatism·A I Sapolsky, D S Howell
Sep 4, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Yuko YokoyamaPeter Brossart
Feb 1, 1996·The International Journal of Biochemistry & Cell Biology·C L Wilson, L M Matrisian
Oct 18, 1983·Biochimica Et Biophysica Acta·T I MoralesJ F Woessner
Jan 1, 1985·The International Journal of Biochemistry·J S Bond, R J Beynon
Aug 31, 1987·Biochemical and Biophysical Research Communications·H A Farach, W J Strittmatter

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