The F-box protein Fbxo7 interacts with human inhibitor of apoptosis protein cIAP1 and promotes cIAP1 ubiquitination

Biochemical and Biophysical Research Communications
Yung-Fu ChangChung-Yee Yuo

Abstract

Human cIAP1 protein is a member of the inhibitor of apoptosis proteins (IAPs) that are involved in apoptosis regulation and an increasing number of other functions, including cell cycle and intracellular signal transduction. In order to identify novel proteins involved in cIAP1 regulation, we performed a yeast two-hybrid screen and identified an F-box protein Fbxo7 as a cIAP1 interacting protein. Co-immunoprecipitation assay showed that cIAP1 can interact with Fbxo7 in human cells. When co-expressed in cells, cIAP1 and Fbxo7 co-localized remarkably both in the cytoplasm and nucleus, and considerable amounts of these often co-localized at one or few distinct Golgi-like structures close to the nucleus. Furthermore, we showed that overexpression of Fbxo7 promotes the ubiquitination of cIAP1. Since F-box proteins are specificity determining subunits of SCF ubiquitin protein ligases, our results suggest that Fbxo7 can mediate the ubiquitination of cIAP1 by SCF ubiquitin protein ligase and thus have important implication in the regulation of cIAP1 function.

References

Jul 17, 1997·Nature·Q L DeverauxJ C Reed
May 9, 1998·The Journal of Biological Chemistry·R TakahashiJ C Reed
Oct 6, 1998·Annual Review of Biochemistry·A Hershko, A Ciechanover
Feb 17, 1999·Journal of Biochemistry·F Yamao
Feb 17, 1999·Genes & Development·Q L Deveraux, J C Reed
Oct 26, 1999·Current Biology : CB·C CenciarelliM Pagano
Oct 26, 1999·Current Biology : CB·J T WinstonJ W Harper
Dec 28, 1999·Annual Review of Cell and Developmental Biology·R J Deshaies
Feb 24, 2001·Genome Biology·E T Kipreos, M Pagano
Mar 27, 2001·Nature Reviews. Molecular Cell Biology·A M Weissman
Mar 22, 2002·Nature·Xiaoming LiJonathan D Ashwell
Apr 19, 2002·Nature·Ning ZhengNikola P Pavletich
Jun 4, 2002·Nature Reviews. Molecular Cell Biology·Guy S Salvesen, Colin S Duckett
Jan 15, 2003·The Journal of Biological Chemistry·Shimin Hu, Xiaolu Yang
Jul 17, 2003·The Journal of Biological Chemistry·Ramesh HegdeEmad S Alnemri
Nov 25, 2003·Oncogene·Peter ListonRobert G Korneluk
Apr 2, 2005·Nature Reviews. Molecular Cell Biology·David L Vaux, John Silke

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Citations

Aug 13, 2013·Nature Neuroscience·Victoria S BurchellHelene Plun-Favreau
Oct 25, 2011·Nature Reviews. Cancer·Michael J DevineNicholas W Wood
Aug 13, 2013·Nucleic Acids Research·Gautier StollOlivier Delattre
Oct 11, 2013·Open Biology·David E NelsonHeike Laman
Apr 29, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·Emma RedonPascal Sourdaine
Sep 21, 2010·Neurogenetics·Lucio SantoroVincenzo Bonifati
Feb 11, 2016·The International Journal of Biochemistry & Cell Biology·Nana ZhengWenyi Wei
Aug 28, 2015·Human Molecular Genetics·Zhi Dong ZhouEng-King Tan
Nov 29, 2011·Journal of Cellular and Molecular Medicine·Elena RadiAntonio Federico
Jan 4, 2012·Journal of Cellular and Molecular Medicine·Hendrik J KuikenAnnette M Dirac
Jan 29, 2013·Neurobiology of Aging·Chin-Hsien LinRuey-Meei Wu
Apr 9, 2010·Retrovirology·Anna Bergamaschi, Gianfranco Pancino
Aug 1, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Yung-Fu ChangChao-Neng Tseng
May 10, 2016·Biochimica Et Biophysica Acta·Nana ZhengWenyi Wei
Dec 13, 2016·Genome Génome / Conseil National De Recherches Canada·Eric M MerzettiBrian E Staveley
Jan 15, 2014·Acta Crystallographica. Section D, Biological Crystallography·Jinsai ShangZhihua Du
Dec 30, 2011·Neuro-degenerative Diseases·Silvia A MandelMoussa B H Youdim
Mar 25, 2014·Nature Reviews. Cancer·Zhiwei WangWenyi Wei
Mar 7, 2019·PloS One·Carmen Ballesteros ReviriegoAnneliese O Speak
Aug 10, 2016·The Biochemical Journal·Felipe Roberti TeixeiraHeike Laman
Dec 5, 2016·Cellular and Molecular Life Sciences : CMLS·Shachi P PatelHeike Laman
Oct 9, 2013·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Jinsai ShangZhihua Du
Aug 17, 2020·Cell Death & Disease·Xiaohui ZhuLi Liang
May 23, 2008·The Journal of Biological Chemistry·Rebecca KirkNeil Q McDonald
Apr 15, 2017·The Biochemical Journal·Helen Walden, Miratul M K Muqit
Jul 16, 2008·Journal of Proteome Research·Jian WangFuchu He
Oct 4, 2020·Biochimica Et Biophysica Acta. General Subjects·Valentine SpagnolFelipe Roberti Teixeira

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