The faciogenital dysplasia gene product FGD1 functions as a Cdc42Hs-specific guanine-nucleotide exchange factor.

The Journal of Biological Chemistry
Y ZhengY Xu

Abstract

The Rho family of small GTP-binding proteins plays important roles in the regulation of actin cytoskeleton organization and cell growth. Activation of these GTPases involves the replacement of bound GDP with GTP, a process catalyzed by the Dbl-like guanine-nucleotide exchange factors, all of which seem to share a putative catalytic motif termed the Dbl homology (DH) domain, followed by a pleckstrin homology (PH) domain. Here we have examined the role of a Dbl-like molecule, the faciogenital dysplasia gene product (FGD1), which when mutated in its Dbl homology domain, cosegregates with the developmental disease Aarskog-Scott syndrome. We report that a polypeptide of FGD1 encompassing the DH and PH domains can bind specifically to the Rho family GTPase Cdc42Hs and stimulates the GDP-GTP exchange of the isoprenylated form of Cdc42Hs. Microinjection of this FGD1 polypeptide into Swiss 3T3 fibroblast cells induces the formation of peripheral actin microspikes, similar to that previously observed when cells were injected with a constitutively active form of Cdc42Hs. This effect of FGD1 on actin organization is readily inhibited by coinjection of a dominant-negative mutant of Cdc42Hs. Examination of NIH 3T3 cells expressing the FGD1 f...Continue Reading

References

Jan 1, 1995·Methods in Enzymology·R A CerioneY Zheng
Jan 1, 1995·Methods in Enzymology·Y ZhengR A Cerione
Nov 24, 1995·The Journal of Biological Chemistry·S BagrodiaR A Cerione
Sep 29, 1995·The Journal of Biological Chemistry·S BagrodiaR A Cerione
Oct 13, 1995·The Journal of Biological Chemistry·S ZhangG M Bokoch
Jul 28, 1995·The Journal of Biological Chemistry·Z ZhuJ Li
Apr 21, 1995·The Journal of Biological Chemistry·Y ZhengD Toksoz
May 23, 1995·Proceedings of the National Academy of Sciences of the United States of America·L StowersJ Chant
Dec 1, 1994·Trends in Genetics : TIG·N Lamarche, A Hall
Jan 1, 1994·Annual Review of Cell Biology·A Hall
Dec 16, 1993·Nature·M S Boguski, F McCormick
Apr 1, 1996·Current Opinion in Cell Biology·R A Cerione, Y Zheng

❮ Previous
Next ❯

Citations

Oct 26, 2000·American Journal of Medical Genetics·J P FrynsA Vianna-Morgante
Jul 25, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·J L GorskiZ Liu
Feb 13, 2007·Neuroradiology·Michael L DilunaMurat Gunel
May 10, 2002·Trends in Neurosciences·Ger J A Ramakers
Apr 12, 2002·Gene·Benjamin Boettner, Linda Van Aelst
Nov 21, 2002·Trends in Cell Biology·Matthew Buechner
Dec 10, 1998·Nature Structural Biology·B AghazadehM K Rosen
Jul 9, 2002·Molecular Microbiology·Gerhard WeinzierlMichael Bölker
Jun 1, 1997·Molecular Biology of the Cell·C G BurdS D Emr
Oct 6, 1997·Molecular Biology of the Cell·T H ChuangG M Bokoch
Sep 27, 2006·Molecular and Cellular Biology·Elena V KostenkoIan P Whitehead
Jan 3, 2001·Molecular and Cellular Biology·K ZhuY Zheng
Sep 7, 2002·Molecular and Cellular Biology·Li ChengIan P Whitehead
Jun 4, 2013·Annual Review of Cell and Developmental Biology·Matthew B GreenblattLaurie H Glimcher
Oct 4, 2011·The Journal of Clinical Investigation·Weiguo ZouLaurie H Glimcher
Dec 17, 2011·PloS One·Yves A LussierRalph R Weichselbaum
Aug 31, 2001·Biochemical and Biophysical Research Communications·W IkedaY Takai
Jun 9, 2001·Biochemical and Biophysical Research Communications·Y FurukawaY Nakamura
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·P D BurbeloS Spiegel
Mar 19, 2014·Cell and Tissue Research·Joana Amado-AzevedoGeerten P Van Nieuw Amerongen
Dec 3, 2010·Journal of Neurodevelopmental Disorders·Liana KaufmanJohn B Vincent
Jul 11, 2008·Cell Motility and the Cytoskeleton·Amanda Gatesman Ammer, Scott A Weed
Feb 18, 2011·Genesis : the Journal of Genetics and Development·Pilar Cejudo-Martin, Sara A Courtneidge
May 11, 2005·Progress in Neurobiology·Iryna M Ethell, Elena B Pasquale
Apr 4, 2008·Developmental Biology·Xiangyan Tong, Matthew Buechner
Jun 15, 2007·American Journal of Human Genetics·Claudia StendelJan Senderek
Mar 2, 2011·The American Journal of Pathology·Lin GaoChristopher S Chen
Apr 8, 2009·International Review of Cell and Molecular Biology·Fernando Martín-Belmonte, Alejo E Rodríguez-Fraticelli

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.