The fallopian tube microbiome: implications for reproductive health

Oncotarget
Elise S PelzerFlavia Huygens

Abstract

There is a paucity of data characterizing the microbiota of the female upper genital tract, which controversially is described as a sterile site. We examine whether the fallopian tube harbours an endogenous microbial community. This prospective study collected from women undergoing total hysterectomy or salpingectomy-oophorectomy. Private hospital gynaecology department. Fallopian tubes were collected from women diagnosed with benign disease or for prophylaxis. Samples were interrogated for the presence of microbial DNA using a next generation sequencing technology approach to exploit the V5 to V9 regions of the 16S rRNA gene. The fallopian tube microbiota was characterized using traditional culture techniques and next generation sequencing. Bacteria were isolated from 50% of cultured samples, and 100% of samples returned positive PCR results. Only 68% of the culture isolates could be confidently identified using automated diagnostic equipment in a clinical microbiology laboratory. Monomicrobial communities were identified only for cultured isolates (50%). Pyrosequencing revealed that all communities were polymicrobial. Lactobacillus spp. were not present in all groups, nor were they the most dominant isolates. Distinct differe...Continue Reading

References

Dec 1, 1989·European Journal of Obstetrics, Gynecology, and Reproductive Biology·D MeirowJ G Schenker
Dec 1, 1983·The Journal of Infectious Diseases·C E SwensonJ Schachter
Mar 1, 1995·Acta Obstetricia Et Gynecologica Scandinavica·B R MøllerS C Mogensen
Nov 11, 2003·Fertility and Sterility·Kenny D Maslow, Edward A Lyons
Jun 23, 2005·International Journal of STD & AIDS·S StepanovićL Seifert
Aug 2, 2005·International Journal of Gynaecology and Obstetrics : the Official Organ of the International Federation of Gynaecology and Obstetrics·S R SinghalS K Singhal
Mar 30, 2007·Journal of Microbiological Methods·Soumitesh ChakravortyDavid Alland
Feb 26, 2008·Applied and Environmental Microbiology·Jeremy A FrankGary J Olsen
Mar 14, 2008·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Alexandre BricouCharles Chapron
Apr 11, 2009·PLoS Computational Biology·James Robert WhiteMihai Pop
Jul 8, 2009·Mikrobiologiia·N A GolodG I El'-Registan
Jun 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jacques RavelLarry J Forney
Dec 24, 2011·Applied and Environmental Microbiology·Kuan-Liang LiuGary Xie
Jan 27, 2012·Journal of Clinical Microbiology·Alan J WolfeLinda Brubaker
May 1, 2012·Nature Methods·Lauren BraggGene W Tyson
May 4, 2012·Science Translational Medicine·Pawel GajerJacques Ravel
Jul 4, 2012·Annual Review of Microbiology·Bing MaJacques Ravel
Nov 28, 2012·Current Protocols in Microbiology·Justin KuczynskiRob Knight
Mar 26, 2013·Liver International : Official Journal of the International Association for the Study of the Liver·Alexandra AlexopoulouDimitrios Pectasides
Jul 5, 2013·Clinical Microbiology Reviews·Itzhak BrookEllie J C Goldstein
Sep 17, 2013·Applied and Environmental Microbiology·Thomas K WoodBrian W Kwan
May 23, 2014·Science Translational Medicine·Kjersti AagaardJames Versalovic
Apr 15, 2017·Oncotarget·Sagarika BanerjeeErle S Robertson
Jan 5, 2018·Antonie van Leeuwenhoek·Elise S PelzerFlavia Huygens

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Citations

Sep 27, 2018·Future Microbiology·Elise S PelzerMelissa Buttini
Oct 27, 2018·Journal of Assisted Reproduction and Genetics·Noble K Kurian, Deepak Modi
Jul 22, 2019·BJOG : an International Journal of Obstetrics and Gynaecology·E Rosenberg, I Zilber-Rosenberg
Mar 17, 2020·Current Opinion in Obstetrics & Gynecology·Sam Schoenmakers, Joop Laven
Jan 16, 2020·Genes·Jessica L O'CallaghanElise S Pelzer
Sep 11, 2018·Frontiers in Immunology·Signe Altmäe
Dec 14, 2018·Frontiers in Immunology·Jonathan D Lenz, Joseph P Dillard
Jan 19, 2019·F1000Research·Jörn Bullerdiek, Birgit Rommel
Aug 3, 2019·International Journal of Molecular Sciences·Adriana PericMilos Stojanov
Aug 8, 2020·Frontiers in Cellular and Infection Microbiology·Lucia RiganelliFrancesca Guerrieri
Feb 19, 2021·Human Reproduction·Alberto Sola-LeyvaSigne Altmäe
Dec 12, 2020·Journal of Gynecology Obstetrics and Human Reproduction·O TsonisM Paschopoulos
May 30, 2021·Trends in Endocrinology and Metabolism : TEM·Madeline E GrahamMarc J Tetel
Jun 11, 2021·Journal of Assisted Reproduction and Genetics·Paula Punzón-Jiménez, Elena Labarta

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Methods Mentioned

BETA
454 sequencing
hysterectomy
dissection
PCR

Software Mentioned

Acacia
VITEK
BLAST
custom Perl script
R gplots library
Metastats
QIIME

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