PMID: 36432May 1, 1979

The fate of IgE bound to rat basophilic leukemia cells

The Journal of Immunology : Official Journal of the American Association of Immunologists
C IserskyT Triche

Abstract

The present study investigates the fate of the cell-bound IgE by using a well-characterized rat basophilic leukemia cell line and a purifed IgE myeloma protein. Both histamine-releasing and nonreleasing cell lines were examined. In both cases, no evidence for cell-mediated IgE catabolism could be elicited. Both the dissociated IgE and the receptors remained intact for prolonged periods of time, as demonstrated by binding assays. Internalization and/or recycling of membrane-bound IgE could not be demonstrated by E. M. autoradiography. We found only limited time-dependent changes in accessibility to anti-IgE antibody, trypsin, or elution at low pH (2.9 to 3.1). A biphasic dissociation of cell-bound 125I-IgE during incubation in the presence of excess unlabeled IgE was reproducibly observed; the more slowly dissociated IgE was also less readily dissociated at pH 3.4. These studies lead us to conclude that, in vitro, IgE resides in a functional orientation on the surface of RBL-1 cells, for prolonged periods of time.

Related Concepts

Autoradiography
Basophils
Binding Sites, Antibody
Neoplastic Cell Transformation
Hydrogen-Ion Concentration
Immunoglobulin E
Leukemia, Experimental
Trypure

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